Isolation and Identification of Swine hlfIuenza Virus

Abstract：Three H3 subtype strains of Swine influenza virus(SlV)，which were named SSH1，SFJ1 and SHB1，were isolated from Shanghai，Fujian and Hubei．These isolates could grow and replicate in chicken emb~o，and the hemagglutinin(HA)values were 1：2 ，1：2 and 1：2 ，respectively．The hemagglutinin inhibitor(HI)titers of H subtype antiserum were 1：2 ，1：2 and 1：2。，respectively, whereas they were negative in HI assays with Newcastle disease an tiserum．Under electron micrograph， the virions showed various shapes．The mice infected witl1 allantoic fluid could arise syndromes an d pathology；SIV could be isolated again and amplified by RT-PCR from the lung．RNA Was extracted from SIV which Was purified from infected allantoic fluid，an d expected ban d Was observed via RT—PCR．