Citation: WU Rui, JIN Mei—fin, CHEN Huan-chuen, WANG Guei-hua, JIANG Zeng-hai. Isolation and Identification of Swine hlfIuenza Virus .VIROLOGICA SINICA, 2003, 18(6) : 553-556.

Isolation and Identification of Swine hlfIuenza Virus

  • Available online: 25 December 2003
  • Abstract:Three H3 subtype strains of Swine influenza virus(SlV),which were named SSH1,SFJ1 and SHB1,were isolated from Shanghai,Fujian and Hubei.These isolates could grow and replicate in chicken emb~o,and the hemagglutinin(HA)values were 1:2 ,1:2 and 1:2 ,respectively.The hemagglutinin inhibitor(HI)titers of H subtype antiserum were 1:2 ,1:2 and 1:2。,respectively, whereas they were negative in HI assays with Newcastle disease an tiserum.Under electron micrograph, the virions showed various shapes.The mice infected witl1 allantoic fluid could arise syndromes an d pathology;SIV could be isolated again and amplified by RT-PCR from the lung.RNA Was extracted from SIV which Was purified from infected allantoic fluid,an d expected ban d Was observed via RT—PCR.

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    Isolation and Identification of Swine hlfIuenza Virus

    • 1. Animal Virus Lab, Animal Science and Veterinary College,HuazhongAgricultural University,Wuhan4307~ China

    Abstract: Abstract:Three H3 subtype strains of Swine influenza virus(SlV),which were named SSH1,SFJ1 and SHB1,were isolated from Shanghai,Fujian and Hubei.These isolates could grow and replicate in chicken emb~o,and the hemagglutinin(HA)values were 1:2 ,1:2 and 1:2 ,respectively.The hemagglutinin inhibitor(HI)titers of H subtype antiserum were 1:2 ,1:2 and 1:2。,respectively, whereas they were negative in HI assays with Newcastle disease an tiserum.Under electron micrograph, the virions showed various shapes.The mice infected witl1 allantoic fluid could arise syndromes an d pathology;SIV could be isolated again and amplified by RT-PCR from the lung.RNA Was extracted from SIV which Was purified from infected allantoic fluid,an d expected ban d Was observed via RT—PCR.

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