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Volume 18 Issue 6
December 2003
    Citation: AN Xue-fang, LIU Feng-song, FANG Ming-gang, ZHU You-, WANG Han-zhong. Isolation and Identification of a M ouse Poxvirus an d Its Infectivit .VIROLOGICA SINICA, 2003, 18(6) : 563-565.
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    Isolation and Identification of a M ouse Poxvirus an d Its Infectivit

    • 1.

      The Key Laboratory of Molecular Virology,Wuhan lnsititute of Virology,The Chinese Academy ofSciences,Wuhan 430071。China 2．Experimental Animal Center,Yunyang Medical College in Hubei Province,Shiyan 442000,China

    • Available online: 25 December 2003
    • Mousepoxvirus has been isolated from diferent pathological tissues in deed mouse that natively infected by virus．In negative stained preparations．the size of the virus particles Was l60～ 19Ohm X250~ 300nm and the virus the particle revealed ovoid·shape．Tl1e healthy Blab／C mouse were infected by injection with purified virus particles，resulting in the death of mouse and producing the same virus particles as inoculated virus．Ttle electron microscopy of an ultrathin section confirmed that viral replication an d assembly of virions occurred in the cytoplasm of diferent pathological tissues infec ted by viruses such as liver,lung．kidney,intestine an d pan creas．These results demonstratedⅡlat the virus should been classified into Poxviridae．
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      Isolation and Identification of a M ouse Poxvirus an d Its Infectivit

      • 1. The Key Laboratory of Molecular Virology,Wuhan lnsititute of Virology,The Chinese Academy ofSciences,Wuhan 430071。China 2．Experimental Animal Center,Yunyang Medical College in Hubei Province,Shiyan 442000,China

      Abstract: Mousepoxvirus has been isolated from diferent pathological tissues in deed mouse that natively infected by virus．In negative stained preparations．the size of the virus particles Was l60～ 19Ohm X250~ 300nm and the virus the particle revealed ovoid·shape．Tl1e healthy Blab／C mouse were infected by injection with purified virus particles，resulting in the death of mouse and producing the same virus particles as inoculated virus．Ttle electron microscopy of an ultrathin section confirmed that viral replication an d assembly of virions occurred in the cytoplasm of diferent pathological tissues infec ted by viruses such as liver,lung．kidney,intestine an d pan creas．These results demonstratedⅡlat the virus should been classified into Poxviridae．

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