Citation: CHENG Rui, LIANG Chang, NAN Fang, HU Zhi, VLAK M, CHEN Xin-wen. Helicoverpa armigera Nucleopolyhedrovirus Triggered Se-UCR Apoptosis is Blocked by Spodoptera exigua Nucleopolyhedrovirus .VIROLOGICA SINICA, 2003, 18(6) : 587-592.

Helicoverpa armigera Nucleopolyhedrovirus Triggered Se-UCR Apoptosis is Blocked by Spodoptera exigua Nucleopolyhedrovirus

  • Available online: 25 December 2003
  • Abstract:Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV)and Spodoptera exigua muUicapsid nucleopolyhedrovirus(SeMNPV)are commercially produced as pesticides.As pe sticides,both viruses have very narrow host range and Can not make cross infection.Trying tO generate a rec ombinant baculovirus with broader host range by CO—infection and co-transfection,we found that HaSNPV could trigger apoptosis of a exigua cell line Se·UCR.which could be blocked by SeM NPV,but apoptosis did not occur in Se一30 1,a cell line also from exigua.W hen infected Se-UCR with 5M OI,theapoptoticbodywasIn’stfound at 12hpi,obvious apoptosis couldbe observedat 24hpi, at the end almost all ceHs were dead at 72 hpi.Those observations were confu-med by genome DNA fragmen-ration analysis.Dot blot an alysis indicated that SeMNPV could also help HaSNPV genome DNA an d then virus repficafion in Se-UCR cells,but not in Se-301.

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    Helicoverpa armigera Nucleopolyhedrovirus Triggered Se-UCR Apoptosis is Blocked by Spodoptera exigua Nucleopolyhedrovirus

    • 1. 1.KeyLab MokcuI口r vimlogy.WuhanInstituteofvimlogy.ChineseAcademyofSciences,Wuhan430071.China
    • 2. Lab of vimlogy.Wageningen University.Binnenhaven 11。6709PD Wageningen,the Netherlands

    Abstract: Abstract:Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV)and Spodoptera exigua muUicapsid nucleopolyhedrovirus(SeMNPV)are commercially produced as pesticides.As pe sticides,both viruses have very narrow host range and Can not make cross infection.Trying tO generate a rec ombinant baculovirus with broader host range by CO—infection and co-transfection,we found that HaSNPV could trigger apoptosis of a exigua cell line Se·UCR.which could be blocked by SeM NPV,but apoptosis did not occur in Se一30 1,a cell line also from exigua.W hen infected Se-UCR with 5M OI,theapoptoticbodywasIn’stfound at 12hpi,obvious apoptosis couldbe observedat 24hpi, at the end almost all ceHs were dead at 72 hpi.Those observations were confu-med by genome DNA fragmen-ration analysis.Dot blot an alysis indicated that SeMNPV could also help HaSNPV genome DNA an d then virus repficafion in Se-UCR cells,but not in Se-301.

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