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Volume 19 Issue 1
February 2004
    Citation: HUA Qun-yi, ZHANG Nian-zu, DONG Jun, YANG Jing-yan, XU Zi-zhong, YANG Yun-qin. Cloning and Sequence Analysis of N gene encoding Akabane virus Nucleotide Capsid Protein .VIROLOGICA SINICA, 2004, 19(1) : 18-21.
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    Cloning and Sequence Analysis of N gene encoding Akabane virus Nucleotide Capsid Protein

    • 1.

      1. Technology Center of Yunnan Entry-Exit Inspection and Quarantine, Kunming 650228, China

    • 2.

      The key Opening Laboratory of Tropical and Subtropical Animal Virology of Ministry of Agriculture, Kunming 650224, China

    • Corresponding author: HUA Qun-yi, 
    • Available online: 20 February 2004
    • The nucleocapsid protein (N) gene of Akabane virus strain YN isolated from bovine was amplified by RT-PCR. The product of 696 bp fragment was obtained as expected. The amplified fragment was then cloned into the pDM18-T vector and confirmed by PCR, endonuclease analysis, and sequencing. The results demonstrated that nucleotide homology with other published AKAV N genes was between 94.2%~98.3%, and the deduced amino acid homology was between 97.6%~99.5%, respectively. Therefore, nucleotide capsid protein gene of AKAV was conserved. Phylogenetic tree constructed with 17 reference strains of 696 bp of the N gene showed that AKAV-YN strain is related to Tinaroo strain.
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      Cloning and Sequence Analysis of N gene encoding Akabane virus Nucleotide Capsid Protein

        Corresponding author: HUA Qun-yi,
      • 1. 1. Technology Center of Yunnan Entry-Exit Inspection and Quarantine, Kunming 650228, China
      • 2. The key Opening Laboratory of Tropical and Subtropical Animal Virology of Ministry of Agriculture, Kunming 650224, China

      Abstract: The nucleocapsid protein (N) gene of Akabane virus strain YN isolated from bovine was amplified by RT-PCR. The product of 696 bp fragment was obtained as expected. The amplified fragment was then cloned into the pDM18-T vector and confirmed by PCR, endonuclease analysis, and sequencing. The results demonstrated that nucleotide homology with other published AKAV N genes was between 94.2%~98.3%, and the deduced amino acid homology was between 97.6%~99.5%, respectively. Therefore, nucleotide capsid protein gene of AKAV was conserved. Phylogenetic tree constructed with 17 reference strains of 696 bp of the N gene showed that AKAV-YN strain is related to Tinaroo strain.

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