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Volume 19 Issue 1
February 2004
    Citation: WANG Chun-ping, ZHU Jie-qing, KANG Yun, George F, TIEN Po. Cloning and Expression of Heptad Repeat Region of the Feline immunodeficiency virus and Product Purification .VIROLOGICA SINICA, 2004, 19(1) : 22-26.
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    Cloning and Expression of Heptad Repeat Region of the Feline immunodeficiency virus and Product Purification

    • 1.

      1. Modern Virology Laboratory, College of Life Science, Wuhan University, Wuhan 430072, China

    • 2.

      Department of Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China

    • 3.

      Nu.eld Department of Clinical Medicine, John Radcli.e Hospital, University of Oxford, Oxford OX3 9DU, UK

    • Corresponding author: TIEN Po, 
    • Available online: 20 February 2004
    • By using a computational program, Learn Coil-VMF, we determined the two conserved heptad repeat regions (HR1 and HR2) of FIV gp40 protein. The gene of gp40 subdomain including HR1 and HR2 was constructed with PCR method. The HR1 and HR2 were subsequently expressed and purified as a single chain (named 2-Helix) connected by a six aminoacid linker in E. coli GST fusion expression system. The single HR1 and HR2 were also cloned and expressed in the same way. Subsequently the 2-Helix′s formation of oligomer was tested. The results showed that the HR1 and HR2 interacted with each other and formed an oligomer.
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      Cloning and Expression of Heptad Repeat Region of the Feline immunodeficiency virus and Product Purification

        Corresponding author: TIEN Po,
      • 1. 1. Modern Virology Laboratory, College of Life Science, Wuhan University, Wuhan 430072, China
      • 2. Department of Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China
      • 3. Nu.eld Department of Clinical Medicine, John Radcli.e Hospital, University of Oxford, Oxford OX3 9DU, UK

      Abstract: By using a computational program, Learn Coil-VMF, we determined the two conserved heptad repeat regions (HR1 and HR2) of FIV gp40 protein. The gene of gp40 subdomain including HR1 and HR2 was constructed with PCR method. The HR1 and HR2 were subsequently expressed and purified as a single chain (named 2-Helix) connected by a six aminoacid linker in E. coli GST fusion expression system. The single HR1 and HR2 were also cloned and expressed in the same way. Subsequently the 2-Helix′s formation of oligomer was tested. The results showed that the HR1 and HR2 interacted with each other and formed an oligomer.

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