Cloning and Expression of Heptad Repeat Region of the Feline immunodeficiency virus and Product Purification
Abstract: By using a computational program, Learn Coil-VMF, we determined the two conserved heptad repeat regions (HR1 and HR2) of FIV gp40 protein. The gene of gp40 subdomain including HR1 and HR2 was constructed with PCR method. The HR1 and HR2 were subsequently expressed and purified as a single chain (named 2-Helix) connected by a six aminoacid linker in E. coli GST fusion expression system. The single HR1 and HR2 were also cloned and expressed in the same way. Subsequently the 2-Helix′s formation of oligomer was tested. The results showed that the HR1 and HR2 interacted with each other and formed an oligomer.