Citation: NIU Guo-dong, ZHANG Zhong-xin*. Interaction between Ubiquitin and IAP2 or IAP3 of Spodptera exigua Nucleopolyhedrovirus .VIROLOGICA SINICA, 2004, 19(1) : 49-53.

Interaction between Ubiquitin and IAP2 or IAP3 of Spodptera exigua Nucleopolyhedrovirus

  • Available online: 20 February 2004
  • By using the yeast two hybrid system, in vitro assay for interaction between ubiquitin and IAP2 or IAP3 encoded by Spodptera exigua nucleopolyhedrovirus(SeNPV)has been done. The putative positive clones only existed on the plate of the SD/-Leu/-Trp/X-α-GAL, which suggested that ubiquitin interacted with IAP2 or IAP3 weakly, and ubiquitin was transferred to IAP2 or IAP3 through ubiquitin-activating enzyme (E1) and ubiquitin-conjugating enzyme (E2) in yeast. IAPs encoded by SeNPV may be the ubiquitin ligase (E3).

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    Interaction between Ubiquitin and IAP2 or IAP3 of Spodptera exigua Nucleopolyhedrovirus

    • 1. Key Laboratory of Molecular Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

    Abstract: By using the yeast two hybrid system, in vitro assay for interaction between ubiquitin and IAP2 or IAP3 encoded by Spodptera exigua nucleopolyhedrovirus(SeNPV)has been done. The putative positive clones only existed on the plate of the SD/-Leu/-Trp/X-α-GAL, which suggested that ubiquitin interacted with IAP2 or IAP3 weakly, and ubiquitin was transferred to IAP2 or IAP3 through ubiquitin-activating enzyme (E1) and ubiquitin-conjugating enzyme (E2) in yeast. IAPs encoded by SeNPV may be the ubiquitin ligase (E3).

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