Citation: ZHU Jin, TAO Kai-hua, CAO Min, ZHANG Yun, LI Yue-xi*, HANG Jing-hai, TANG Jia-qi. Establishment of Diagnostic Assay for Hantavirus with Microarray Techniques .VIROLOGICA SINICA, 2004, 19(1) : 79-81.

Establishment of Diagnostic Assay for Hantavirus with Microarray Techniques

  • Available online: 20 February 2004
  • To establish a rapid, sensitive and specific diagnostic assay for Hantavirus with microarray techniques, specific primers and probes were designed according to the conservative and specific DNA sequence of 76-118 strain and R22 strain. The probes were spotted on glass slides to form microarrays. The Cy3-labled single stranded DNA fragments prepared by dissymmetical PCR were hybridized with the probes on the glass slides. The microarrays were scanned and analyzed with a scanner. The results showed that the DNA microarray could detect the different typed DNA of HTN and SEO with adequate specificity and sensitivity. The developed DNA microarray and techniques might be a very useful method for diagnosis and prevention, and could be widely applied in specific pathogens detection of infectious diseases such as hemorrhagic fever with renal syndrome.

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    Establishment of Diagnostic Assay for Hantavirus with Microarray Techniques

    • 1. Nanjing Military Institute, Nanjing Command, Nanjing 210002, China

    Abstract: To establish a rapid, sensitive and specific diagnostic assay for Hantavirus with microarray techniques, specific primers and probes were designed according to the conservative and specific DNA sequence of 76-118 strain and R22 strain. The probes were spotted on glass slides to form microarrays. The Cy3-labled single stranded DNA fragments prepared by dissymmetical PCR were hybridized with the probes on the glass slides. The microarrays were scanned and analyzed with a scanner. The results showed that the DNA microarray could detect the different typed DNA of HTN and SEO with adequate specificity and sensitivity. The developed DNA microarray and techniques might be a very useful method for diagnosis and prevention, and could be widely applied in specific pathogens detection of infectious diseases such as hemorrhagic fever with renal syndrome.

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