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Volume 19 Issue 2
April 2004
    Citation: KONG Xiao-hong, YU Hong, XUAN Cheng-hao, WANG Jin-zhong, CHEN Qi-min, GENG Yun-qi*, . Regulation of RU5 Region to Gene Expression of BFV 3026 .VIROLOGICA SINICA, 2004, 19(2) : 129-132.
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    Regulation of RU5 Region to Gene Expression of BFV 3026

    • 1.

      College of Life Sciences, Nankai University, Tianjin 300071, China

    • Available online: 20 April 2004
    • In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.
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      Regulation of RU5 Region to Gene Expression of BFV 3026

      • 1. College of Life Sciences, Nankai University, Tianjin 300071, China

      Abstract: In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.

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