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Volume 19 Issue 2
April 2004
    Citation: LIU Shu-ying, MA Xue-en, LI Jing-peng, ZHANG Jiu-he. Partial Cloning and Sequence Analysis of the gag Gene of Jaagsiekte Sheep Retrovirus NM Strain .VIROLOGICA SINICA, 2004, 19(2) : 133-136.
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    Partial Cloning and Sequence Analysis of the gag Gene of Jaagsiekte Sheep Retrovirus NM Strain

    • 1.

      1. College of Animal Science and Medicine, Inner Mongolia Agricultural University, Huhhot 010018, China

    • 2.

      College of life science, Northeast Agricultural University, Harbin 150030, China

    • Corresponding author: LIU Shu-ying, 
    • Available online: 20 April 2004
    • In order to amplify partial gag gene of Jaagsiekte sheep retrovirus Inner Mongolia strain, a pair of primers was designed according to the GenBank sequence . The fragment of gag gene was obtained by polymerase chain reaction (PCR), and the gene was cloned into PMD-18 T vector and identified by EcoR I and Sal I digestion. The nucleotide and amino acid sequences of NM strain partial gag gene were compared with the counterpart sequeuces of South Africa strain (Accession No. NC-001494) and USA strain (Accession No. AF105220). The nucleotide and amino acid homology of partial gag gene were 83%, 81.5% and 84%, 83%, respectively. This is the first JSRV nucleotide sequence reported in China.
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      Partial Cloning and Sequence Analysis of the gag Gene of Jaagsiekte Sheep Retrovirus NM Strain

        Corresponding author: LIU Shu-ying,
      • 1. 1. College of Animal Science and Medicine, Inner Mongolia Agricultural University, Huhhot 010018, China
      • 2. College of life science, Northeast Agricultural University, Harbin 150030, China

      Abstract: In order to amplify partial gag gene of Jaagsiekte sheep retrovirus Inner Mongolia strain, a pair of primers was designed according to the GenBank sequence . The fragment of gag gene was obtained by polymerase chain reaction (PCR), and the gene was cloned into PMD-18 T vector and identified by EcoR I and Sal I digestion. The nucleotide and amino acid sequences of NM strain partial gag gene were compared with the counterpart sequeuces of South Africa strain (Accession No. NC-001494) and USA strain (Accession No. AF105220). The nucleotide and amino acid homology of partial gag gene were 83%, 81.5% and 84%, 83%, respectively. This is the first JSRV nucleotide sequence reported in China.

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