Citation: JIN Feng-mei, LIN Li-ming, LIN Qi-ying*, XIE Lian-hui. Regeneration of Transgenic Rice with the Rice Grassy Stunt Virus sp Gene .VIROLOGICA SINICA, 2004, 19(2) : 146-148.

Regeneration of Transgenic Rice with the Rice Grassy Stunt Virus sp Gene

  • Available online: 20 April 2004
  • Rice grassy stunt virus sp gene was transformed into rice by Agrobacterium mediated transformation. PCR analysis and Southern blot hybridization by using the genomic DNA of regenerated plants showed that the sp gene was transformed into rice. By using RT-PCR Southern blot hybridization, it was confirmed that the sp gene had been transtripted into mRNA.

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    Regeneration of Transgenic Rice with the Rice Grassy Stunt Virus sp Gene

    • 1. Institute of Plant Virology, Fujian Agricultural and Foresrtry University, Fuzhou 350002,China

    Abstract: Rice grassy stunt virus sp gene was transformed into rice by Agrobacterium mediated transformation. PCR analysis and Southern blot hybridization by using the genomic DNA of regenerated plants showed that the sp gene was transformed into rice. By using RT-PCR Southern blot hybridization, it was confirmed that the sp gene had been transtripted into mRNA.

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