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Volume 19 Issue 2
April 2004
    Citation: ZHANG Su-fang, JIA Yun, WANG Min-xiu, NI Yan-xiu, HE Kong-wang, CHEN Pu-yan. Establishment of Nested RT-PCR Diagnostic Method for Porcine Epidemic Diarrhea Virus .VIROLOGICA SINICA, 2004, 19(2) : 174-176.
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    Establishment of Nested RT-PCR Diagnostic Method for Porcine Epidemic Diarrhea Virus

    • 1.

      1. Key Laboratory of Animal Disease diagnosis and Immunology, Ministry of Agriculture at Nanjing Agricultural University, Nanjing 210095

    • 2.

      Department of veterinary medicine, Shenyang Agricultural University, Shenyang 110161

    • 3.

      Key Lab of Animal and Poultry Diseases Diagnostic, Ministry of Agriculture at jiangsu Academy of Agricultural Science, Nanjing, 210014

    • Corresponding author: HE Kong-wang, 
    • Available online: 20 April 2004
    • Two pairs of primers were designed according to the N gene sequence of PEDV-CV777 strain in Genebank (No. AF353511). PCR amplification product of outer-primers was 1328bp, and the inter-primers amplification product was 538bp. With the primers, a nested PCR assay was established to detect PEDV. This method was sensitive and specific and could be used in PEDV diagnosis and epidemiological investigation.
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      Establishment of Nested RT-PCR Diagnostic Method for Porcine Epidemic Diarrhea Virus

        Corresponding author: HE Kong-wang,
      • 1. 1. Key Laboratory of Animal Disease diagnosis and Immunology, Ministry of Agriculture at Nanjing Agricultural University, Nanjing 210095
      • 2. Department of veterinary medicine, Shenyang Agricultural University, Shenyang 110161
      • 3. Key Lab of Animal and Poultry Diseases Diagnostic, Ministry of Agriculture at jiangsu Academy of Agricultural Science, Nanjing, 210014

      Abstract: Two pairs of primers were designed according to the N gene sequence of PEDV-CV777 strain in Genebank (No. AF353511). PCR amplification product of outer-primers was 1328bp, and the inter-primers amplification product was 538bp. With the primers, a nested PCR assay was established to detect PEDV. This method was sensitive and specific and could be used in PEDV diagnosis and epidemiological investigation.

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