Citation: HU Jun, DONG Chang-yuan*, WANG Fan-jun, CHEN Xiao, GNO Shu-fang, ZHANG Wei-ying. The Study of Amplifing HCV ssRNA by RT-PCR Combined With Transcription in vitro .VIROLOGICA SINICA, 2004, 19(3) : 214-216.

The Study of Amplifing HCV ssRNA by RT-PCR Combined With Transcription in vitro

  • Available online: 20 June 2004
  • The purpose of this study is to establish a new method which HCV RNA can be used to detect sensitively and specifically through efficient single strand RNAs(ssRNAs) amplification. The sera from the chronic Hepatitis C patients were used as specimens. Single step reverse transcription-PCR (SRT-PCR) was performed prospectively, with a designed set of primers which defined the sequence unique to the 5’ noncoding region of the HCV genome, a great deal of specific ssRNAs were synthesized in the presence of T7RNA polymerase and other appropriate conditions within five hours. Comparing with the nested-PCR, the quantity of the products in our method was nearly 20-fold higher and its specificity was verified by the electrophoresis on agarose gel and the dot hybridization with the probe.

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    The Study of Amplifing HCV ssRNA by RT-PCR Combined With Transcription in vitro

    • 1. Lab of Molecular Virus & Cancer, Institute of Virology, Medical College, Wuhan University, Wuhan 430071, China

    Abstract: The purpose of this study is to establish a new method which HCV RNA can be used to detect sensitively and specifically through efficient single strand RNAs(ssRNAs) amplification. The sera from the chronic Hepatitis C patients were used as specimens. Single step reverse transcription-PCR (SRT-PCR) was performed prospectively, with a designed set of primers which defined the sequence unique to the 5’ noncoding region of the HCV genome, a great deal of specific ssRNAs were synthesized in the presence of T7RNA polymerase and other appropriate conditions within five hours. Comparing with the nested-PCR, the quantity of the products in our method was nearly 20-fold higher and its specificity was verified by the electrophoresis on agarose gel and the dot hybridization with the probe.

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