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Volume 19 Issue 3
June 2004
    Citation: LU Yu-rong, FENG Zong-di, WU Dong, SUN Xiu-lian, DENG Fei, YUAN Li, HU Zhi-hong. Study of Foreign Baculoviral ctl Gene by Overexpression Using AcMNPV Bacmid System in vivo .VIROLOGICA SINICA, 2004, 19(3) : 276-280.
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    Study of Foreign Baculoviral ctl Gene by Overexpression Using AcMNPV Bacmid System in vivo

    • 1.

      1.The key laboratory of Molecular Virology and Joint-lab of Invertebrate Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071 China

    • 2.

      Department of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, the Netherlands

    • Corresponding author: HU Zhi-hong, 
    • Available online: 20 June 2004
    • The conotoxinlike (ctl) gene is a small gene in the genomes of several baculoviruses. The biological function of baculoviral ctl gene remains unknown. In this report, we generated a recombinant AcMNPV(AcBac-ph-ctl) which contains the ctl gene from BusuNPV using AcMNPV bacmid system. The transcription of ctl gene by AcBac-ph-ctl was confirmed by RT-PCR in infected Sf21 cells. To study the CTL function in vivo, the recombinant viruses were used to infect Spodotera exigua peros, and the infectivity and action speed were determined. No significant difference between the median lethal concentration (LC50) and the median survival time (ST50) of the wild type virus AcMNPV and the recombinant virus was found .
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      Study of Foreign Baculoviral ctl Gene by Overexpression Using AcMNPV Bacmid System in vivo

        Corresponding author: HU Zhi-hong,
      • 1. 1.The key laboratory of Molecular Virology and Joint-lab of Invertebrate Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071 China
      • 2. Department of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, the Netherlands

      Abstract: The conotoxinlike (ctl) gene is a small gene in the genomes of several baculoviruses. The biological function of baculoviral ctl gene remains unknown. In this report, we generated a recombinant AcMNPV(AcBac-ph-ctl) which contains the ctl gene from BusuNPV using AcMNPV bacmid system. The transcription of ctl gene by AcBac-ph-ctl was confirmed by RT-PCR in infected Sf21 cells. To study the CTL function in vivo, the recombinant viruses were used to infect Spodotera exigua peros, and the infectivity and action speed were determined. No significant difference between the median lethal concentration (LC50) and the median survival time (ST50) of the wild type virus AcMNPV and the recombinant virus was found .

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