Citation: XU Fang-ling, YANG Zhan-qiu*, CHENG Li, YANG Chi-Chiang, LIU Han-yan, XIAO Hong, WEN Li, DING Xiao-hua, CHEN Wen-kai. Selection of Differentially Expressed Signal Transduction-Associated Genes of Integrin in Hantavirus Infected Cells .VIROLOGICA SINICA, 2004, 19(4) : 315-319.

Selection of Differentially Expressed Signal Transduction-Associated Genes of Integrin in Hantavirus Infected Cells

  • Available online: 25 August 2004
  • To develop the pathogenesis of Hantavirus infection, the differentially expressed signal trans- duction-associated genes of integrin in Hantavirus infected cells were investigated by using cDNA microarray. The primary human embryo pulmonary fibroblasts (HEPFs) were infected with Hantaan 76-118 virus. Total RNAs were extracted from infected and normal control group 6h, 24h and 96h post infection, respectively. During reverse transcription, the cDNAs were labeled by Cy5-dUTP and Cy3-dUTP. The labeled cDNAs hybridized with the Biostar-ⅡH gene chip. After hybridization, the fluorescence signal was analyzed by scanning. It showed that the expression level of most (113/150) Hantavirus receptor and signal transduction-associated genes was normal, however, 37 genes presented obvious change. Among them, expressions of 31 genes changed at 6h post infection. 13 genes were down-regulated, and 18 up-regulated. However, 5 and 4 genes expressed diversely at 24h and 96h post infection respectively. Furthermore, some integrin signal transduction pathway-associated genes such as the gene of some related protein kinases were up-regulated at 6h post infection. The gene of enhancer of filamentation 1 was down-regulated at 6h and 96h post infection. The gene of microtubule-associated protein 1A was up-regulated at 6h post infection, but down-regulated at 24h and 96h post infection. These indicate that Hantavirus infection is related to integrins.

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    Selection of Differentially Expressed Signal Transduction-Associated Genes of Integrin in Hantavirus Infected Cells

    • 1. Laboratory of Virology of Ministry of Education, Institute of Virology, Medical School, Wuhan University, Wuhan 430071, China

    Abstract: To develop the pathogenesis of Hantavirus infection, the differentially expressed signal trans- duction-associated genes of integrin in Hantavirus infected cells were investigated by using cDNA microarray. The primary human embryo pulmonary fibroblasts (HEPFs) were infected with Hantaan 76-118 virus. Total RNAs were extracted from infected and normal control group 6h, 24h and 96h post infection, respectively. During reverse transcription, the cDNAs were labeled by Cy5-dUTP and Cy3-dUTP. The labeled cDNAs hybridized with the Biostar-ⅡH gene chip. After hybridization, the fluorescence signal was analyzed by scanning. It showed that the expression level of most (113/150) Hantavirus receptor and signal transduction-associated genes was normal, however, 37 genes presented obvious change. Among them, expressions of 31 genes changed at 6h post infection. 13 genes were down-regulated, and 18 up-regulated. However, 5 and 4 genes expressed diversely at 24h and 96h post infection respectively. Furthermore, some integrin signal transduction pathway-associated genes such as the gene of some related protein kinases were up-regulated at 6h post infection. The gene of enhancer of filamentation 1 was down-regulated at 6h and 96h post infection. The gene of microtubule-associated protein 1A was up-regulated at 6h post infection, but down-regulated at 24h and 96h post infection. These indicate that Hantavirus infection is related to integrins.

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