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Volume 19 Issue 4
August 2004
    Citation: LIU Yan-hua, FAN Wei-xing, WANG Xi-le, LIU Wen-qiang, XU Chuan-tian, ZHANG Zhi. Cloning and Sequence Analysis of NS1 Gene of Porcine Parvovirus SD-68 Strain .VIROLOGICA SINICA, 2004, 19(4) : 353-355.
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    Cloning and Sequence Analysis of NS1 Gene of Porcine Parvovirus SD-68 Strain

    • 1.

      1.College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China

    • 2.

      China Animal Epizooti- ology Center, Qingdao 266032, China

    • Corresponding author: FAN Wei-xing, 
    • Available online: 25 August 2004
    • NS1 gene of Porcine parvovirus SD-68 strain was cloned and sequenced. Based on the sequence we can conclude that NS1 gene of SD-68 strain is composed of 1989 nucleotides, encodes a polypiptide of 662 amino acids and shares 99.9%, 99.9%, 99.7%, 98.1% identity of nucleotides and 99.7%, 99.5%, 99.5%, 96.7% identity of amino acids with that of SY-99 strain, Kresse strain, NADL-2(5075) strain and NADL-2(4973) strain, respectively. The distances of NS1 gene of all strains analyzed by phylogenetic tree, we can conclude that SD-68 strain is near to MVM(i) strain and far to BPV3 strains. The residues of Thr and Ser at the position 435 and 473 in the NS1 gene of SD-68 strain are phosphorylated sites because they are also found at the same position in the NS1 gene of MVM(i) strain.
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      Cloning and Sequence Analysis of NS1 Gene of Porcine Parvovirus SD-68 Strain

        Corresponding author: FAN Wei-xing,
      • 1. 1.College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
      • 2. China Animal Epizooti- ology Center, Qingdao 266032, China

      Abstract: NS1 gene of Porcine parvovirus SD-68 strain was cloned and sequenced. Based on the sequence we can conclude that NS1 gene of SD-68 strain is composed of 1989 nucleotides, encodes a polypiptide of 662 amino acids and shares 99.9%, 99.9%, 99.7%, 98.1% identity of nucleotides and 99.7%, 99.5%, 99.5%, 96.7% identity of amino acids with that of SY-99 strain, Kresse strain, NADL-2(5075) strain and NADL-2(4973) strain, respectively. The distances of NS1 gene of all strains analyzed by phylogenetic tree, we can conclude that SD-68 strain is near to MVM(i) strain and far to BPV3 strains. The residues of Thr and Ser at the position 435 and 473 in the NS1 gene of SD-68 strain are phosphorylated sites because they are also found at the same position in the NS1 gene of MVM(i) strain.

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