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Volume 19 Issue 4
August 2004
    Citation: DENG Yu-xiu, JIANG Ping, LI Yu-feng, WANG Xian-wei, JIANG Wen-ming, TANG Jing-yuan. Analysis of Porcine Reproductive and Respiratory Syndrome Virus Isolates from Eastern China with RT-PCR-RFLP .VIROLOGICA SINICA, 2004, 19(4) : 398-400.
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    Analysis of Porcine Reproductive and Respiratory Syndrome Virus Isolates from Eastern China with RT-PCR-RFLP

    • 1.

      Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agriculture University, Nanjing 210095,China

    • Corresponding author: JIANG Ping, 
    • Available online: 25 August 2004
    • Porcine reproductive and respiratory syndrome virus(PRRSV) isolates identified in samples from 50 field cases originated from eastern China herds were studied. ORF5 gene of PRRSV isolates was amplified by reverse transcript polymerase chain reaction(RT-PCR) and restriction fragment length polymorphism (RFLP) patterns for enzymes MluⅠ, HincⅡand SacⅡwere determined on these ORF5 genes. The RFLP pattern obtained from 39 isolates was 2-2-1(78%), 3 isolates was 1-1-1(6%) and 8 isolates identified in 2003 was 1-3-1 (16%). The results showed PRRSV strains have variations and existed no less than 3 gene subtypes in the area.
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      Analysis of Porcine Reproductive and Respiratory Syndrome Virus Isolates from Eastern China with RT-PCR-RFLP

        Corresponding author: JIANG Ping,
      • 1. Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agriculture University, Nanjing 210095,China

      Abstract: Porcine reproductive and respiratory syndrome virus(PRRSV) isolates identified in samples from 50 field cases originated from eastern China herds were studied. ORF5 gene of PRRSV isolates was amplified by reverse transcript polymerase chain reaction(RT-PCR) and restriction fragment length polymorphism (RFLP) patterns for enzymes MluⅠ, HincⅡand SacⅡwere determined on these ORF5 genes. The RFLP pattern obtained from 39 isolates was 2-2-1(78%), 3 isolates was 1-1-1(6%) and 8 isolates identified in 2003 was 1-3-1 (16%). The results showed PRRSV strains have variations and existed no less than 3 gene subtypes in the area.

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