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Volume 19 Issue 5

October 2004

Citation: LI Lin, YING Tian-yi, ZHUANG Dao-min, BAO Zuo-yi, LIU Si-yang, LI Han-ping, LIU Yong-jian, YANG Kun, LI Jue, WANG Heng-liang, HUANG Liu-yu, LI Jing-yun. Study on the Different Expression Proteins of MT4 Cells Infected by HIV-1 ⅢB Strain .VIROLOGICA SINICA, 2004, 19(5) : 435-438.

Study on the Different Expression Proteins of MT4 Cells Infected by HIV-1 ⅢB Strain

LI Lin ¹ ,
YING Tian-yi ² ,
ZHUANG Dao-min ¹ ,
BAO Zuo-yi ¹ ,
LIU Si-yang ¹ ,
LI Han-ping ¹ ,
LIU Yong-jian ¹ ,
YANG Kun ¹ ,
LI Jue ¹ ,
WANG Heng-liang ² ,
HUANG Liu-yu ² ,
LI Jing-yun ^{1*
,,}

1.
1. Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing 100071, China
2.
Institute of Biotechnology, Academy of Military Medical Science, Beijing 100071, China

Corresponding author: LI Jing-yun,
Available online: 20 October 2004

Abstract

Abstract：To analyze the diferent expression proteins of MT4 cells infected witl1 HIV 1 IIIB strain．The proteins of MT4 cells from infected and Uninfected HIV 1 IIIB were separated bv 2DE and the gels were an alyzed with software Image Master 2D Elite 3．01 to find out the diferent spots．The proteins in the spots were identified by matrix associated laser desorption／ionization time．of-flight(MALDI．TOF) mass spectrometry．A total of 40 diferent protein spots were found．1 2 proteins were down-regulated in the M T4 cells after infection；the other 28 proteins were up．regulated．29 proteins were detected bv M ALDI—T0E Th e proteins that down．regulated in MT4 cells after infecteion include proteins related to energy metabolism，actin proteins an d hypothetical proteins．The up—regulated proteins in MT4 cells after infecteion include proteins related to actin proteins，enzymes．immune proteins an d hypothetical proteins．In the M T4 cells infected bv HI man y proteins were chan ged．In order to understand the mechanism of infection of HIV,we must wipe Of the abundan t proteins an d study the proteins witl1 special functions．
- MT4
- , HIV
- , Two-dimensional electrophoresis
- , Peptide mass fingerprinting

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Study on the Different Expression Proteins of MT4 Cells Infected by HIV-1 ⅢB Strain

Corresponding author: LI Jing-yun,

1. 1. Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing 100071, China
2. Institute of Biotechnology, Academy of Military Medical Science, Beijing 100071, China

Abstract: Abstract：To analyze the diferent expression proteins of MT4 cells infected witl1 HIV 1 IIIB strain．The proteins of MT4 cells from infected and Uninfected HIV 1 IIIB were separated bv 2DE and the gels were an alyzed with software Image Master 2D Elite 3．01 to find out the diferent spots．The proteins in the spots were identified by matrix associated laser desorption／ionization time．of-flight(MALDI．TOF) mass spectrometry．A total of 40 diferent protein spots were found．1 2 proteins were down-regulated in the M T4 cells after infection；the other 28 proteins were up．regulated．29 proteins were detected bv M ALDI—T0E Th e proteins that down．regulated in MT4 cells after infecteion include proteins related to energy metabolism，actin proteins an d hypothetical proteins．The up—regulated proteins in MT4 cells after infecteion include proteins related to actin proteins，enzymes．immune proteins an d hypothetical proteins．In the M T4 cells infected bv HI man y proteins were chan ged．In order to understand the mechanism of infection of HIV,we must wipe Of the abundan t proteins an d study the proteins witl1 special functions．

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