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Volume 19 Issue 5
October 2004
    Citation: JIA Yun, LU Yin-hua, ZHANG Su-fang, ZHOU Bin, CHEN Pu-yan. Investigation on Epidemiology of Co-infection of Porcine Circovirus Type 2, Porcine Reproductive and Respiratory Disease Syndrome Virus and Porcine Parvovirus .VIROLOGICA SINICA, 2004, 19(5) : 467-470.
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    Investigation on Epidemiology of Co-infection of Porcine Circovirus Type 2, Porcine Reproductive and Respiratory Disease Syndrome Virus and Porcine Parvovirus

    • 1.

      1. Key Laboratory of Animal Disease Diagnosis and Immunology, Ministry of Agriculture at Nanjing Agricultural University, Nanjing, 210095 China

    • 2.

      Department of Veterinary Medicine, Shenyang Agricultural University, Shenyang 1101611, China.

    • Corresponding author: CHEN Pu-yan, 
    • Available online: 20 October 2004
    • According to the published genomic sequences of Porcine reproductive and respiratory disease syndrome virus (PRRSV)，Porcine parvovirus (PPV) and Porcine circovirus type 2, primers were designed and RT-PCR, PCR and multiplex PCR were set up for the detection of PRRSV, PPV and PCV, respectively. 127 samples were detected for PCV by the multiplex PCR. With the established methods, 67 clinical samples positive for porcine circovirus type 2 (PCV2) were tested for the presence of PRRSV and PPV. The results demonstrated that the co-infection rate of PRRSV/PCV2, PPV/PCV2 and PRRSV/PPV/PCV2 were detected in 52.3% (35/67), 26.9% (18/67) and 7.5% (5/67), respectively. The data obtained in the present study indicated that it is very prevalent for the co-infection of PRRSV, PPV and PCV2.
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      Investigation on Epidemiology of Co-infection of Porcine Circovirus Type 2, Porcine Reproductive and Respiratory Disease Syndrome Virus and Porcine Parvovirus

        Corresponding author: CHEN Pu-yan,
      • 1. 1. Key Laboratory of Animal Disease Diagnosis and Immunology, Ministry of Agriculture at Nanjing Agricultural University, Nanjing, 210095 China
      • 2. Department of Veterinary Medicine, Shenyang Agricultural University, Shenyang 1101611, China.

      Abstract: According to the published genomic sequences of Porcine reproductive and respiratory disease syndrome virus (PRRSV)，Porcine parvovirus (PPV) and Porcine circovirus type 2, primers were designed and RT-PCR, PCR and multiplex PCR were set up for the detection of PRRSV, PPV and PCV, respectively. 127 samples were detected for PCV by the multiplex PCR. With the established methods, 67 clinical samples positive for porcine circovirus type 2 (PCV2) were tested for the presence of PRRSV and PPV. The results demonstrated that the co-infection rate of PRRSV/PCV2, PPV/PCV2 and PRRSV/PPV/PCV2 were detected in 52.3% (35/67), 26.9% (18/67) and 7.5% (5/67), respectively. The data obtained in the present study indicated that it is very prevalent for the co-infection of PRRSV, PPV and PCV2.

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