Citation: FAN Chang-fa, GONG Wei, MEI Xing-guo, LIU Yan. Cloning of Gene Encoding G Protein from Respiratory Syncytial Virus and Co-expression of G Protein Fragments with Diverse Carrier Proteins .VIROLOGICA SINICA, 2004, 19(6) : 553-558.

Cloning of Gene Encoding G Protein from Respiratory Syncytial Virus and Co-expression of G Protein Fragments with Diverse Carrier Proteins

  • Corresponding author: MEI Xing-guo, 
  • Available online: 20 December 2004
  • Although severe diseases may be caused by respiratory syncytial virus (RSV) infection in the world, no efficacious vaccine against this virus is licensed. In an effort to seek recombinant protein antigens that may be used in the future vaccine development, we constructed a series of expression vectors which can co-express carrier proteins and G protein fragments based on cloning the full cDNA sequence of G protein from RSV. An expression system that can efficiently express recombinant protein antigens in soluble form was selected for subsequent researches. Balb/c mice were immunized with one of the purified recombinant protein antigens, DsbA-G101, for production of anti- serum. Based on ELISA assays, good immunogenicity of DsbA-G101 was revealed. The constructed expression vectors can be used in immunogenicity analysis of different G protein fragments and in selection of ideal carrier proteins, two aspects which are important for the future development of vaccine against this virus.

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    Cloning of Gene Encoding G Protein from Respiratory Syncytial Virus and Co-expression of G Protein Fragments with Diverse Carrier Proteins

      Corresponding author: MEI Xing-guo,
    • 1. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, 27 Taiping Road, Beijing 100850, China

    Abstract: Although severe diseases may be caused by respiratory syncytial virus (RSV) infection in the world, no efficacious vaccine against this virus is licensed. In an effort to seek recombinant protein antigens that may be used in the future vaccine development, we constructed a series of expression vectors which can co-express carrier proteins and G protein fragments based on cloning the full cDNA sequence of G protein from RSV. An expression system that can efficiently express recombinant protein antigens in soluble form was selected for subsequent researches. Balb/c mice were immunized with one of the purified recombinant protein antigens, DsbA-G101, for production of anti- serum. Based on ELISA assays, good immunogenicity of DsbA-G101 was revealed. The constructed expression vectors can be used in immunogenicity analysis of different G protein fragments and in selection of ideal carrier proteins, two aspects which are important for the future development of vaccine against this virus.

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