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Volume 19 Issue 6
December 2004
    Citation: LIU Yan-hua, FAN Wei-xing, SUI Zhao-feng, WANG Xi-le, XU Long-tao, SUN Zhong-cheng, GAO Bao-shan. Cloning and Sequence Analysis of vp2 Gene of Porcine Parvovirus SD-68 Strain .VIROLOGICA SINICA, 2004, 19(6) : 568-571.
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    Cloning and Sequence Analysis of vp2 Gene of Porcine Parvovirus SD-68 Strain

    • 1.

      1.College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China

    • 2.

      China Animal Epizootiology Center, Qingdao 266032, China

    • 3.

      Qilu Animal Health Products Factory, Jinan 250001, China

    • Corresponding author: FAN Wei-xing, 
    • Available online: 20 December 2004
    • vp2 gene of Porcine parvovirus (PPV) SD-68 strain was cloned and sequenced .The results showed that vp2 gene of Porcine parvovirus (PPV) SD-68 strain was 1740bp and encoded a protein of 579 amino acids. vp2 gene of SD-68 strain exhibited over 99% nucleotide sequence identity and 96% amino acid sequence identity with other PPV strains: Kresse, SY-99, NADL-2(5075), NADL-2(4973), US-1. Phylogenetic tree analysis of vp2 gene showed the closest relationship between SD-68 strain and kresse strain. The difference between SD-68 strain and kresse strain was only on 378, 383 and 436 residues, which decided the tissue tropism of PPV, so the tissue tropism of SD-68 strain suggested more similar with kresse stain, that is to say, SD-68 was a dermatitis strain. Comparison of amino acid residues of virulent strain (kreese) and vaccine strain (NADL-2 and SD-68) suggested that 215, 378 and 383 were important residues that decided the pathogenicity of PPV.
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      Cloning and Sequence Analysis of vp2 Gene of Porcine Parvovirus SD-68 Strain

        Corresponding author: FAN Wei-xing,
      • 1. 1.College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
      • 2. China Animal Epizootiology Center, Qingdao 266032, China
      • 3. Qilu Animal Health Products Factory, Jinan 250001, China

      Abstract: vp2 gene of Porcine parvovirus (PPV) SD-68 strain was cloned and sequenced .The results showed that vp2 gene of Porcine parvovirus (PPV) SD-68 strain was 1740bp and encoded a protein of 579 amino acids. vp2 gene of SD-68 strain exhibited over 99% nucleotide sequence identity and 96% amino acid sequence identity with other PPV strains: Kresse, SY-99, NADL-2(5075), NADL-2(4973), US-1. Phylogenetic tree analysis of vp2 gene showed the closest relationship between SD-68 strain and kresse strain. The difference between SD-68 strain and kresse strain was only on 378, 383 and 436 residues, which decided the tissue tropism of PPV, so the tissue tropism of SD-68 strain suggested more similar with kresse stain, that is to say, SD-68 was a dermatitis strain. Comparison of amino acid residues of virulent strain (kreese) and vaccine strain (NADL-2 and SD-68) suggested that 215, 378 and 383 were important residues that decided the pathogenicity of PPV.

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