Citation: WANG Fu-xiang, SUN Yong-tao, LIU Juan, MA Ying-ji, YANG Bao-shan, YAN Bing-zhu. Interleukin-12 Gene Modulates Immune Responses Induced by HIV-1 Nucleic Acid Vaccine .VIROLOGICA SINICA, 2005, 20(1) : 16-19.

Interleukin-12 Gene Modulates Immune Responses Induced by HIV-1 Nucleic Acid Vaccine

  • Available online: 20 January 2005
  • To investigate the effect of interleukin-12 DNA immunization on immune responses induced by HIV-1 nucleic acid vaccine(DNA vaccine)and to explore new strategies for therapeutic HIV-1 DNA vaccine, Balb/c mice were immunized with pCI-neoGAG alone or co-administered with the DNA encoding for interleukin-12 (IL-12).Their sera were collected for analyzing anti-HIV antibody and IFN-γ by ELISA , and splenocytes were isolated for detecting antigen-specific lymphoproliferative responses and specific CTL response by MTT assay and LDH assay respectively.Our research showed that the anti-HIV antibody titers of mice co-immunized with pCI-neoGAG and the DNA encoding for interleukin-12 (IL-12) were lower than that of mice immunized with pCI-neoGAG alone(P0.01); In contrast ,the IFN-γ level of mice co-immunized with pCI-neoGAG and the DNA encoding for interleukin-12 was higher than that of mice immunized with pCI-neoGAG alone(P0.01).Furthermore, compared with mice injected with pCI-neoGAG alone, the specific CTL cytotoxity

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    Interleukin-12 Gene Modulates Immune Responses Induced by HIV-1 Nucleic Acid Vaccine

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    Abstract: To investigate the effect of interleukin-12 DNA immunization on immune responses induced by HIV-1 nucleic acid vaccine(DNA vaccine)and to explore new strategies for therapeutic HIV-1 DNA vaccine, Balb/c mice were immunized with pCI-neoGAG alone or co-administered with the DNA encoding for interleukin-12 (IL-12).Their sera were collected for analyzing anti-HIV antibody and IFN-γ by ELISA , and splenocytes were isolated for detecting antigen-specific lymphoproliferative responses and specific CTL response by MTT assay and LDH assay respectively.Our research showed that the anti-HIV antibody titers of mice co-immunized with pCI-neoGAG and the DNA encoding for interleukin-12 (IL-12) were lower than that of mice immunized with pCI-neoGAG alone(P0.01); In contrast ,the IFN-γ level of mice co-immunized with pCI-neoGAG and the DNA encoding for interleukin-12 was higher than that of mice immunized with pCI-neoGAG alone(P0.01).Furthermore, compared with mice injected with pCI-neoGAG alone, the specific CTL cytotoxity

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