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Volume 20 Issue 1
February 2005
    Citation: WANG Shu, WANG Hua-lin, DENG Fei, WU Dong, LONG Gang, YUAN Li, HU Zhi-hong. Construction of a Recombinant HaSNPV Expressing GP64 .VIROLOGICA SINICA, 2005, 20(1) : 79-83.
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    Construction of a Recombinant HaSNPV Expressing GP64

    • 1.

    • Corresponding author: HU Zhi-hong, 
    • Available online: 20 January 2005
    • There are two kinds of membrane fusion proteins encoded by baculovirus: GP64 and F protein. The membrane fusion protein of group I NPV is GP64, while group II NPV is F protein. In this paper. we tried to answer whether GP64 can be expressed and assembled into the BV of Group II NPV. By using Bac-to-Bac system, we successfully constructed recombinant baculoviruses HaSNPVgp64~+egfp~+ possessing GP64 of AcMNPV and EGFP, and HaSNPVegfp~+ with an EGFP as control. Western blotting analysis indicated that GP64 was expressed in HzAM1 cells infected with HaSNPVgp64~+egfp~+ and was packaged into progeny BVs.
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      Construction of a Recombinant HaSNPV Expressing GP64

        Corresponding author: HU Zhi-hong,
      • 1. 

      Abstract: There are two kinds of membrane fusion proteins encoded by baculovirus: GP64 and F protein. The membrane fusion protein of group I NPV is GP64, while group II NPV is F protein. In this paper. we tried to answer whether GP64 can be expressed and assembled into the BV of Group II NPV. By using Bac-to-Bac system, we successfully constructed recombinant baculoviruses HaSNPVgp64~+egfp~+ possessing GP64 of AcMNPV and EGFP, and HaSNPVegfp~+ with an EGFP as control. Western blotting analysis indicated that GP64 was expressed in HzAM1 cells infected with HaSNPVgp64~+egfp~+ and was packaged into progeny BVs.

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