Efficient Pseudotyping of Murine Leukemia Virus Particles with Avian Influenza Virus Hemagglutinin Gly- coproteins

A transient three-plasmid expression system for the production of pseudotyping virions were used to construct the pseudotype of murine leukemia virus(MuLV) with avian influenza virus hemagglutinin(HA) glycoproteins. Co-transfected pcDNA-HA, pHIT60(include MuLV structural genes, namely Gag and Pol) and pHIT111(retroviral genome, containing Lac Z as a reporter) into 293T cells. The retroviral supernatants were harvested 48 hours post-transfection, filtered through a 0.45μM filter. The supernatant were used to analysis the characteristic of the pseudotyping virions by Western blotting and infection test. Western blotting revealed the HA glycoproteins can be expressed on the virions, indicated the HA glycoproteins were incorporated onto the retroviral virions. Infection test were performed on 293T、NIH3T3 and COS-7, all the three kinds of cells infected were Lac Z positive, indicating viral entry, and revealed the pseudotype virions of MuLV-HA were infectious. The pseudotype system of MuLV particles with AIV ˎ̥,Verdana,Arial; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-fareast-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">Hemagglutinin proteins were set up and it can be used to study the ent ry of avian influenza virus isolated f rom goose in China.ˎ̥,Verdana,Arial; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-fareast-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">Hemagglutinin proteins were set up and it can be used to study the ent ry of avian influenza virus isolated f rom goose in China.