Screening of Genes Differentially Expressed in HepG2 Cells Transfected with Gene 1 Transacti- vated by Hepatitis B Virus Pre-s1 with Microarray Assay
Abstract: To understand the differentially expressed target genes in HepG2 cells transfected with PS1TP1 protein expression vector, we compared the differentially expressed genes between the hepatoblastoma cell line HepG2 transfected by pcDNA3.1(-) and pcDNA3.1(-)-PS1TP1, respecti vely with cDNA microarray . The PS1TP1 coding DNA fragment was amplified with polymerase chain reaction (PCR) . The expressive vector of pcDNA3.1(-)-PS1TP1 was constructed by routine molecular biological methods. The HepG2 cells were transfected by pcDNA3.1(-) and pcDNA 3.1(-)-PS1TP1, respectively ,using FuGENE6 Transfection Reagent. Total RNA was isolated and reverse transcribed. The cDNAs were subjected for microarray screening with 4096 cDNA probes. From the scanning results, it was found that 8 genes were up-regulated and 14 genes were down-regulated by PS1TP1 protein expression. The expression of PS1TP1 protein affected the expression spectrum of hepatocyte.