Isolation and Sequence Analysis of S1 Gene of Avian Infectious Bronchitis Virus A Field Strain
Abstract: One strain of Avian Infectious Bronchitis Virus(IBV) was isolated from layer flock which had the symptom of egg drop in Tai’an of Shandong Province, and identified by electron microscope, HA test and animal regressive research. Results of virus neutralization test showed that the isolated virus is a variant strain of IBV. The whole sequence of S1 gene was cloned and analyzed by RT-PCR using specific primers which can differentiate between 793/B IBV and other serotypes of IBV. The strain′s serotype can be primary deduced as 793/B.