Co-expression and Construction of Eukaryote Plasmid of pp38 and pp24 of Marek’s Disease . Virus

pp38 gene and pp24 gene of MDV Md11 strain were cloned into pBudCE(4.1) vector respectively. The pBudCE(4.1)-pp38 and pBudCE(4.1)-pp24 eukaryotic expressing plasmids were transfected into CEF respectively by lipofectamine reagent. 24, 48, 72 hours after transfection, the expression of pp38 was testified with Mab H19, and pp24 with the antiserum of pp24 expressed in E.coli.pp38 gene and pp24 gene were cloned into pBudCE(4.1) vector which can express two different genes at the same time. After transfection,the co-expression of PP38 and PP24 was testified by IFA and by Western-blotting with Anti-GST-pp24 sera.