Detection for CMV,LSV,LMoV Infected Lily with DNA Microarry Techniques

Specific primers and probes were designed according to conservative and specific DNA sequence of CMV,LSV and LMoV.The probes were spotted on glass slides to form microarrays The Cy3-labled single strand DNA fragments prepared by dissymmetical RT-PCR were hybridized with the probes on the glass slides .The microarrays were scanned and analyzed with a scanner. DNA microarray could detect different typed DNA of CMV,LSV and LMoV with adequate specificity and sensitivity . Microarray techniques establish a rapid,sensitive and specific diagnostic assay for lily viruses. The successfully developed DNA microarray and techniques might be a very useful method for diagnosis and prevention ,and widely applied in specific pathogen detection of diseases such as lily virus.