Citation: ZHU Chang-xiang, SONG Yun-zhi, WANG Mei-mei, WANG Xiu-fang, WEN Fu-jiang. Detection for CMV,LSV,LMoV Infected Lily with DNA Microarry Techniques .VIROLOGICA SINICA, 2005, 20(4) : 434-437.

Detection for CMV,LSV,LMoV Infected Lily with DNA Microarry Techniques

  • Available online: 20 August 2005
  • Coat protein (CP) gene of Tobacco ringspot virus (TRSV) Shandong isolate 1 was cloned by reverse transcription-polymerase chain reaction (RT-PCR), and was subcloned into the pET-22b(+) prokaryotic expression vector. The recombinant vector was transformed into E.coli strain BL21. Sequence analysis revealed that the cp gene was 1548 nucleotides in length,encodes a coat protein of 515 amino acids, and shares 90.7%~94.6% nucleotides and amino acid homology with TRSV cp genes registered in GenBank. The target fusion peptide with a molecular weight of 58kDa was expressed under the condition of 23-25℃ and induced by IPTG at a final concentration of 1mmol/L. Rabbit was immunized using the expressed target peptide as antigen, and the antiserum was obtained. The antiserum had a titer of 1/1024 with high specificity to TRSV.

  • 加载中
  • 加载中

Article Metrics

Article views(3952) PDF downloads(949) Cited by()

Related
Proportional views

    Detection for CMV,LSV,LMoV Infected Lily with DNA Microarry Techniques

    • 1. 

    Abstract: Coat protein (CP) gene of Tobacco ringspot virus (TRSV) Shandong isolate 1 was cloned by reverse transcription-polymerase chain reaction (RT-PCR), and was subcloned into the pET-22b(+) prokaryotic expression vector. The recombinant vector was transformed into E.coli strain BL21. Sequence analysis revealed that the cp gene was 1548 nucleotides in length,encodes a coat protein of 515 amino acids, and shares 90.7%~94.6% nucleotides and amino acid homology with TRSV cp genes registered in GenBank. The target fusion peptide with a molecular weight of 58kDa was expressed under the condition of 23-25℃ and induced by IPTG at a final concentration of 1mmol/L. Rabbit was immunized using the expressed target peptide as antigen, and the antiserum was obtained. The antiserum had a titer of 1/1024 with high specificity to TRSV.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return