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Volume 20 Issue 5
October 2005
    Citation: MENG Xian-feng, DAI Xing, MENG Ji-hong, ZHANG Hong-mei, ZHOU Zhen-xian, . Genotype Identification and Variation Analysis of Hepatitis E Virus Isolated in Nanjing, China .VIROLOGICA SINICA, 2005, 20(5) : 468-471.
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    Genotype Identification and Variation Analysis of Hepatitis E Virus Isolated in Nanjing, China

    • 1.

    • Available online: 20 October 2005
    • The objective of this study was to identify and analyze genotype distribution and genetic variation of Hepatitis E virus(HEV) isolated in Nanjing,China.RT-nPCR based on universal primers for multiple HEV genotypes was used to detect HEV RNA in 40 samples collected from patients with acute sporadic hepatitis E.PCR products were sequenced and analyzed with the LASERGENE and PHYLIP softwares.Fourteen out of the 40 samples were positive for RT-nPCR.The positive rate was 35%.All of the fourteen isolates were clustered into HEV genotype IV and were separated into two different sub-genotypes.The nucleotide sequences of the HEV isolates of genotype IV presented higher variability when compared with the sequences of the Chinese isolates of HEV genotype I.Moreover,a new subtype of HEV genotype IV was discovered in this study.
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      Genotype Identification and Variation Analysis of Hepatitis E Virus Isolated in Nanjing, China

      • 1. 

      Abstract: The objective of this study was to identify and analyze genotype distribution and genetic variation of Hepatitis E virus(HEV) isolated in Nanjing,China.RT-nPCR based on universal primers for multiple HEV genotypes was used to detect HEV RNA in 40 samples collected from patients with acute sporadic hepatitis E.PCR products were sequenced and analyzed with the LASERGENE and PHYLIP softwares.Fourteen out of the 40 samples were positive for RT-nPCR.The positive rate was 35%.All of the fourteen isolates were clustered into HEV genotype IV and were separated into two different sub-genotypes.The nucleotide sequences of the HEV isolates of genotype IV presented higher variability when compared with the sequences of the Chinese isolates of HEV genotype I.Moreover,a new subtype of HEV genotype IV was discovered in this study.

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