Serving the Society Since 1986
Advanced Search
Volume 20 Issue 5
October 2005
    Citation: ZHANG Xue-han, HE Kong-wang, GUO Rong-li, NI Yan-fang, WANG Fang, YU Zheng-yu. Cloning and Expression of E Gene of Japanese Encephalitis Virus and Preliminary application .VIROLOGICA SINICA, 2005, 20(5) : 494-497.
    shu

    Cloning and Expression of E Gene of Japanese Encephalitis Virus and Preliminary application

    • 1.

    • Available online: 20 October 2005
    • The whole cDNA of E gene was amplified by RT-PCR from JEV strain SA14-14,and cloned into the pMD18-T vector.The fragment was identified by restriction enzymes digestion with EcoR I and Xho I,cloned into the pET32a(+) vector.The recombinant plasmid was transformed into BL21 and the recombinant bacteria was induced by optimal concentration of IPTG.SDS-PAGE and Western blotting were performed to detect the E fusion protein.Our result indicates the molecular weight of E protein is of E protein 73kDa and its antigenicity is good.Indirect ELISA procedure has been developed with the purified E protein as antigen for detection of JEV.
    • 加载中
    • 加载中
    PDF

    Article Metrics

    Article views(4009) PDF downloads(987) Cited by()

    Related
    Proportional views

      Cloning and Expression of E Gene of Japanese Encephalitis Virus and Preliminary application

      • 1. 

      Abstract: The whole cDNA of E gene was amplified by RT-PCR from JEV strain SA14-14,and cloned into the pMD18-T vector.The fragment was identified by restriction enzymes digestion with EcoR I and Xho I,cloned into the pET32a(+) vector.The recombinant plasmid was transformed into BL21 and the recombinant bacteria was induced by optimal concentration of IPTG.SDS-PAGE and Western blotting were performed to detect the E fusion protein.Our result indicates the molecular weight of E protein is of E protein 73kDa and its antigenicity is good.Indirect ELISA procedure has been developed with the purified E protein as antigen for detection of JEV.

        HTML

      Relative (20)

      目录

      This journal is a member of, and subscribes to the principles of, the Committee on Publication Ethics (COPE)

      鄂ICP备05001977号

         

      鄂公网安备 42010602003674号

      Copyright © 2019 Virologica Sinica. All rights reserved

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return