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Volume 20 Issue 5
October 2005
    Citation: LI Pei, CAO Rui-bing, ZHOU Bin, ZHENG Qi-sheng, WEI Jian-chao, LI Peng, REN Xue-feng, CHEN Fu-yan. Prokaryotic Expression of the GP3 Protein of Porcine Reproductive and Respiratory Syndrome Virus S1 Strain .VIROLOGICA SINICA, 2005, 20(5) : 507-510.
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    Prokaryotic Expression of the GP3 Protein of Porcine Reproductive and Respiratory Syndrome Virus S1 Strain

    • 1.

    • Available online: 20 October 2005
    • The recombinant plasmid pET-VP_2Ⅰ was transformed into BL21 competent cells and expressed in high level after induced with 1.0mmol/l IPTG.The expressed product was purified with His·Bind chromatograghy after being proved by Westen-blot,and was used as an antigen to establish indirect ELISA for detecting antibodies against PPV.The optional working circumstances for the ELISA(antigen concentration 3.5μg/mL;serum dilution 1∶40)were tried out with chess titration.The positive critertion of test serum of this ELISA is OD_490nm0.51,the OD_490 ratio of the tested serum to the negative serum is higher than 2.1.
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      Prokaryotic Expression of the GP3 Protein of Porcine Reproductive and Respiratory Syndrome Virus S1 Strain

      • 1. 

      Abstract: The recombinant plasmid pET-VP_2Ⅰ was transformed into BL21 competent cells and expressed in high level after induced with 1.0mmol/l IPTG.The expressed product was purified with His·Bind chromatograghy after being proved by Westen-blot,and was used as an antigen to establish indirect ELISA for detecting antibodies against PPV.The optional working circumstances for the ELISA(antigen concentration 3.5μg/mL;serum dilution 1∶40)were tried out with chess titration.The positive critertion of test serum of this ELISA is OD_490nm0.51,the OD_490 ratio of the tested serum to the negative serum is higher than 2.1.

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