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Volume 20 Issue 5
October 2005
    Citation: JANG Yi-jun, JIANG Ping*, YANG Xiao-wei, TANG Jing-yuan, LI Yu-feng, LI Yong-dong. Construction and Indentification of Recombinant Adenovirus Containing Multiple Antigen Epitopes of Swine Foot-and-Mouth Disease Virus .VIROLOGICA SINICA, 2005, 20(5) : 511-514.
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    Construction and Indentification of Recombinant Adenovirus Containing Multiple Antigen Epitopes of Swine Foot-and-Mouth Disease Virus

    • 1.

    • Available online: 20 October 2005
    • A recombinant replication defective Human adenovius serotype5 plasmid pAd2VP ,containing amino acids (21260)2(1412160)2(2002213) coding region of swine foot and mouth disease virus serotype O-VP1 , was const ructed using the method of homologous recombination in E.coli BJ5183. After the recombinant plasmid pAd2VP linearized with PacI t ransferred into HEK293A cell , the recombinant virus was isolated and purified in HEK2293A cells by t hree times plaque purification . This recombinant adenovirus could be stably passaged in HEK2293A cells and TCID50 was 10
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      Construction and Indentification of Recombinant Adenovirus Containing Multiple Antigen Epitopes of Swine Foot-and-Mouth Disease Virus

      • 1. 

      Abstract: A recombinant replication defective Human adenovius serotype5 plasmid pAd2VP ,containing amino acids (21260)2(1412160)2(2002213) coding region of swine foot and mouth disease virus serotype O-VP1 , was const ructed using the method of homologous recombination in E.coli BJ5183. After the recombinant plasmid pAd2VP linearized with PacI t ransferred into HEK293A cell , the recombinant virus was isolated and purified in HEK2293A cells by t hree times plaque purification . This recombinant adenovirus could be stably passaged in HEK2293A cells and TCID50 was 10

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