Prokaryotic Expression of the GP3 Protein of Porcine Reproductive and Respiratory Syndrome Virus S1 Strain

The GP3 deleted hydrophobic N-terminal sequence(tGP3) of Porcine reproductive and respiratory syndrome virus(PRRSV) was cloned into prokaryotic expression vector pRSET.The recombinant protein(His)_6-GP3 was highly expressed in E.coli BL21 and could amount to 41% of the total proteins.It could be purified efficiently with affinity chromatography.Western-blotting analysis showed that the recombinant protein was able to react with PRRSV polyclonal antiserum.It can be used for the further investigation on immunogenicity and function of GP3 of PRRSV.