Construction of Hepatitis C Virus E1E2 Envelope Protein Gene DNA Vaccine and Animal Immune Experiment

Hepatitis C virus(HCV) H77 strain E1E2 gene DNA vaccine was constructed by inserting full-length cDNA of HCV E1E2 into an eukaryotic expression vector pcDNA4.0.The recombinant plasmid was transfected into eukaryotic cells 293T by calcium phosphate transfection method and transient expressed E1E2 envelope protein was analyzed by FACS.BALB/c mice were injected intramuscularly with the recombinant plasmid.Anti-HCV E1E2 antibody was detected by FACS with SP2/0 cells which expressed HCV E1E2 protein.Moreover,the antibody was also analyzed by Westrn blot using prolyofic expression E2 protien as the arigen.The results showed that HCV E1E2 protein was expressed transiently in 293T cells.Specific anti-E1E2 antibody could be detected in DNA immune mouse serum by FACS and the antibody could react specifically to SP2/0 cells which express HCV E1E2 protein.Western blot analysis showed that DNA immune mouse serum could react specially to E2 protein expressed in E.coli.