Citation: HE Tao, HU Zhi-hong, FANG Ming-gang, WANG Hua-lin, YANG Ji-hong, WANG Han-zhong. Expression and Purification of Nucleocaspid and Envelope Proteins of SARS Coronavirus .VIROLOGICA SINICA, 2005, 20(6) : 590-593.

Expression and Purification of Nucleocaspid and Envelope Proteins of SARS Coronavirus

  • Available online: 20 December 2005
  • Genes encoding nucleocapsid (N) and envelope (E) proteins of SARS coronavirus (SARS-CoV) were obtained by RT-PCR and were cloned into the expression vector pGEX-KG. The genes were expressed in E. coli and the recombinant proteins were in a soluble form. The proteins were purified by affinity chromatography. The recombinant N protein reacted specifically with anti-SARS antibody. The results may provide a good tool for further research of immune responses and early diagnosis of SARS virus.

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    Expression and Purification of Nucleocaspid and Envelope Proteins of SARS Coronavirus

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    Abstract: Genes encoding nucleocapsid (N) and envelope (E) proteins of SARS coronavirus (SARS-CoV) were obtained by RT-PCR and were cloned into the expression vector pGEX-KG. The genes were expressed in E. coli and the recombinant proteins were in a soluble form. The proteins were purified by affinity chromatography. The recombinant N protein reacted specifically with anti-SARS antibody. The results may provide a good tool for further research of immune responses and early diagnosis of SARS virus.

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