Citation: TONG Xiao, SHEN Tao, LIANG Hua, MENG Qing-lai, ZHONG Wei-zhou, MA Yan, WEI Li-li, XIANG Wen-hua, SHEN Rong-xian, XU Jian-qing, ZHANG Xiao-yan, SHAO Yi-ming. A Novel Flow Cytometric Assay for Evaluating Antigen-specific CTL Responses and Its Application in EIAV Immunological Study .VIROLOGICA SINICA, 2005, 20(6) : 642-646.

A Novel Flow Cytometric Assay for Evaluating Antigen-specific CTL Responses and Its Application in EIAV Immunological Study

  • Available online: 20 December 2005
  • A novel flow cytometric assay for evaluating Equine infectious anemia virus (EIAV) antigen-specific CTL responses was established and by using PKH-26 and CFSE as cell colouration dyes. Compared with the traditional 51Cr -release assay, the new method is more sensitive, has a decrease background and avoids the risk of radioactive exposure to workers. The cellular immuno-response against EIAV infection was detected using this method. The maturation of EIAV antigen-specific CTL response was characterized. The result showed that the CTL response reached a maximum at three months and remained relatively high. The novel method is directly applicable to research on the immune mechanism of the EIAV attenuated vaccine.

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    A Novel Flow Cytometric Assay for Evaluating Antigen-specific CTL Responses and Its Application in EIAV Immunological Study

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    Abstract: A novel flow cytometric assay for evaluating Equine infectious anemia virus (EIAV) antigen-specific CTL responses was established and by using PKH-26 and CFSE as cell colouration dyes. Compared with the traditional 51Cr -release assay, the new method is more sensitive, has a decrease background and avoids the risk of radioactive exposure to workers. The cellular immuno-response against EIAV infection was detected using this method. The maturation of EIAV antigen-specific CTL response was characterized. The result showed that the CTL response reached a maximum at three months and remained relatively high. The novel method is directly applicable to research on the immune mechanism of the EIAV attenuated vaccine.

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