Citation: HU Zhao-li, ZHU Jiang, SHENG Ye, WANG Wen-bing, LI Xin-ping, SHEN Song-dong. Cloning and Expression of the gp41 Gene from SpltMNPV Japan Strain and Analysis of Phylogeny Based on gp41 and ph gene .VIROLOGICA SINICA, 2006, 21(1) : 57-63.

Cloning and Expression of the gp41 Gene from SpltMNPV Japan Strain and Analysis of Phylogeny Based on gp41 and ph gene

  • Corresponding author: ZHU Jiang, 
  • Available online: 20 January 2006
  • The gp41 gene was cloned from Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) Japan-C3 strain. This gene is 993bp long and encodes a polypeptide with a predicted molecular mass of 36.9 kDa. Analysis showed the gp41of SpltMNPV Japan-C3 strain was most closely related to that of SpltMNPV Chinese-G2 strain with 99.9% identity in nucleotide sequence and 99.9% in amino acid sequence. The phylogenetic trees based on gp4l and polyhedrin had the similar topologies. The combined tree of gp41 and polyhedrin had an identical topology as the gp41 gene. Mutation rate analysis showed the gp41 gene had a higher nucleotide substitution rate than that of the polyhedrin gene, implying that the polyhedrin gene may have a different selection constraint than the gp41 gene.

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    Cloning and Expression of the gp41 Gene from SpltMNPV Japan Strain and Analysis of Phylogeny Based on gp41 and ph gene

      Corresponding author: ZHU Jiang,
    • 1. 1. Life Sciences School, Suzhou University, Suzhou, 215006, China
    • 2. Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013, China

    Abstract: The gp41 gene was cloned from Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) Japan-C3 strain. This gene is 993bp long and encodes a polypeptide with a predicted molecular mass of 36.9 kDa. Analysis showed the gp41of SpltMNPV Japan-C3 strain was most closely related to that of SpltMNPV Chinese-G2 strain with 99.9% identity in nucleotide sequence and 99.9% in amino acid sequence. The phylogenetic trees based on gp4l and polyhedrin had the similar topologies. The combined tree of gp41 and polyhedrin had an identical topology as the gp41 gene. Mutation rate analysis showed the gp41 gene had a higher nucleotide substitution rate than that of the polyhedrin gene, implying that the polyhedrin gene may have a different selection constraint than the gp41 gene.

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