Citation: YIN Shuang-hui, LIU Xiang-tao*, HAN Xue-qing, SHANG You-jun, SUN Shi-qi. Expression and Purification of Erns Gene of Classical Swine Fever Virus in E. coli .VIROLOGICA SINICA, 2006, 21(1) : 75-77.

Expression and Purification of Erns Gene of Classical Swine Fever Virus in E. coli

  • Available online: 20 January 2006
  • The Erns gene was amplified by RT-nPCR from C-strain of Classical swine fever virus (CSFV). Then the Erns gene was cloned into pGEX6P-1 vector. The expression products were analysed by SDS-PAGE and Western blotting methods. The inclusion bodies were recovered from bacterial lysate by centrifugation and washed with buffer, and then dissolved in denaturing agents. The protein was refolded by dilution dialysis. Refold protein reacted strongly with the positive serum of CSFV, which indicated that the protein has immunogenic.

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    Expression and Purification of Erns Gene of Classical Swine Fever Virus in E. coli

    • 1. Ministry of Agriculture the People’s Republic of China Key Laboratory of Animal Virology, Lanzhou Veterinary Research Institute, Lanzhou 730046, China

    Abstract: The Erns gene was amplified by RT-nPCR from C-strain of Classical swine fever virus (CSFV). Then the Erns gene was cloned into pGEX6P-1 vector. The expression products were analysed by SDS-PAGE and Western blotting methods. The inclusion bodies were recovered from bacterial lysate by centrifugation and washed with buffer, and then dissolved in denaturing agents. The protein was refolded by dilution dialysis. Refold protein reacted strongly with the positive serum of CSFV, which indicated that the protein has immunogenic.

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