Citation: HU Quan, LEI Yan-chang, ZHANG Zhen-hua, TIAN Yong-jun, ZHANG Zheng-mao, LI Lei, XIA Jian-bo, LU Meng-ji, YANG Dong-liang. Cloning and Sequence Analysis of Duch Hepatitis B virus Genome from Hubei Brown Ducks .VIROLOGICA SINICA, 2006, 21(2) : 163-168.

Cloning and Sequence Analysis of Duch Hepatitis B virus Genome from Hubei Brown Ducks

  • Corresponding author: YANG Dong-liang, 
  • Available online: 20 March 2006
  • Seventy serum samples were collected from adult brown ducks in Hubei were collected in order to clarify the natural Duck hepatitis B virus (DHBV) infection in and to characterize the genome structure of DHBV. The complete genome of the DHBV strain was amplified by polymerase chain reaction (PCR) and cloned into T vector and sequenced. The results showed that the natural infection rate of DHBV in Hubei was 10%. The DHBV genome isolated by PCR (GenBank accession number DQ276978 ) had 3 024 nucleotides with three overlapping reading frames encoding the surface, core and the polymerase proteins, respectively. Comparison of genome sequences of this strain with those of 17 DHBV strains from the GenBank revealed an identity from 89.3% to 93.5% at the nucleotide level. The amino acid sequences of the S protein,core protein,and functional domain of the Pol protein were highly reserved among all of these DHBV strains. This Hubei strain was found to share more signature amino acids in the polymerase genes with the “Chinese” DHBV strains than those of the “Western” country strains. This finding was also corroborated by a phylogenetic tree analysis. Therefore, this Hubei DHBV strain should be classified to a subtype of the Chinese strains.

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    Cloning and Sequence Analysis of Duch Hepatitis B virus Genome from Hubei Brown Ducks

      Corresponding author: YANG Dong-liang,
    • 1. 1. Division of Clinical Immunology,Tongji Hospital of Tongji Medical College,Huazhong University of Science and Technology HUST,Wuhan 430030,China
    • 2. Department of Microbiology, Tongji Medical College of HUST, Wuhan 430030,China
    • 3. Institute of Virology,Medical School of Essen University,Essen 45122, Germany

    Abstract: Seventy serum samples were collected from adult brown ducks in Hubei were collected in order to clarify the natural Duck hepatitis B virus (DHBV) infection in and to characterize the genome structure of DHBV. The complete genome of the DHBV strain was amplified by polymerase chain reaction (PCR) and cloned into T vector and sequenced. The results showed that the natural infection rate of DHBV in Hubei was 10%. The DHBV genome isolated by PCR (GenBank accession number DQ276978 ) had 3 024 nucleotides with three overlapping reading frames encoding the surface, core and the polymerase proteins, respectively. Comparison of genome sequences of this strain with those of 17 DHBV strains from the GenBank revealed an identity from 89.3% to 93.5% at the nucleotide level. The amino acid sequences of the S protein,core protein,and functional domain of the Pol protein were highly reserved among all of these DHBV strains. This Hubei strain was found to share more signature amino acids in the polymerase genes with the “Chinese” DHBV strains than those of the “Western” country strains. This finding was also corroborated by a phylogenetic tree analysis. Therefore, this Hubei DHBV strain should be classified to a subtype of the Chinese strains.

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