Molecular Characterization of Hantavirus Zhejiang Isolate ZT10 Strain
Abstract: The effects of the M gene on the molecular epidemiological characteristics of the Hantavirus ZT10 strain isolated from M. arvalis was investigated. The gene was amplified by RT-PCR and yielded the expected product of 3651 bp. The amplified fragment was then cloned into the T vector and confirmed by PCR and sequencing. Analysis of the sequence revealed that the ZT10 M segment belongs to seoul virus．The nucleotide sequence identity of this M gene with eight virus strains was 84.0%～96.3%. The identity with Hantanvirus（Prospect Hill virus, Tula virus, Khabarovsk virus, Isla vista virus） isolated from arvalis was 57.5%～60.9%. Also, the identiry with the SEO types and with the Guo3 isoated from ZheJiang province was 84.0%. Seoul virus ZT10 strain is a new specific seoul subtype virus in ZheJiang province.