Citation: WENG Jing-qing, XIE Rong-hui, LU Yi-yu, YAO Ping-ping, LI Min-hong, LI Chan, XU Fang, MAO Hai-yan, ZHU Han-ping, CUI Qing-rong, DONG Ying, ZHU Zhi-yong. Molecular Characterization of Hantavirus Zhejiang Isolate ZT10 Strain .VIROLOGICA SINICA, 2006, 21(3) : 235-237.

Molecular Characterization of Hantavirus Zhejiang Isolate ZT10 Strain

  • Corresponding author: WENG Jing-qing, 
  • Available online: 20 May 2006
  • The effects of the M gene on the molecular epidemiological characteristics of the Hantavirus ZT10 strain isolated from M. arvalis was investigated. The gene was amplified by RT-PCR and yielded the expected product of 3651 bp. The amplified fragment was then cloned into the T vector and confirmed by PCR and sequencing. Analysis of the sequence revealed that the ZT10 M segment belongs to seoul virus.The nucleotide sequence identity of this M gene with eight virus strains was 84.0%~96.3%. The identity with Hantanvirus(Prospect Hill virus, Tula virus, Khabarovsk virus, Isla vista virus) isolated from arvalis was 57.5%~60.9%. Also, the identiry with the SEO types and with the Guo3 isoated from ZheJiang province was 84.0%. Seoul virus ZT10 strain is a new specific seoul subtype virus in ZheJiang province.

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    Molecular Characterization of Hantavirus Zhejiang Isolate ZT10 Strain

      Corresponding author: WENG Jing-qing,
    • 1. 1.Zhejiang Provincial Center for Disease Prevention and Control, HangZhou, 310009, China
    • 2. Zhejiang Provincial TanTai Center for Disease Prevention and Control, Zhejiang TanTai, 317200, China

    Abstract: The effects of the M gene on the molecular epidemiological characteristics of the Hantavirus ZT10 strain isolated from M. arvalis was investigated. The gene was amplified by RT-PCR and yielded the expected product of 3651 bp. The amplified fragment was then cloned into the T vector and confirmed by PCR and sequencing. Analysis of the sequence revealed that the ZT10 M segment belongs to seoul virus.The nucleotide sequence identity of this M gene with eight virus strains was 84.0%~96.3%. The identity with Hantanvirus(Prospect Hill virus, Tula virus, Khabarovsk virus, Isla vista virus) isolated from arvalis was 57.5%~60.9%. Also, the identiry with the SEO types and with the Guo3 isoated from ZheJiang province was 84.0%. Seoul virus ZT10 strain is a new specific seoul subtype virus in ZheJiang province.

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