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Volume 21 Issue 3
June 2006
    Citation: WANG Yan, LIU Chun-xin, YI You-rong, CUI Ye-jian, ZHU Jun, DONG Chang-yuan. Detection of Apoptosis of the Human Hepatic Carcinoma Cells Induced by Bluetongus Virus by PI and Annexin V/PI Staining Methods .VIROLOGICA SINICA, 2006, 21(3) : 253-256.
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    Detection of Apoptosis of the Human Hepatic Carcinoma Cells Induced by Bluetongus Virus by PI and Annexin V/PI Staining Methods

    • 1.

      1.Center of Medical Structural Biology, Wuhan University School of Medicine Wuhan University, wuhan 430071, China

    • 2.

      Laboratory of Virus and Cancer, Institute of Virology, Medical College, Wuhan University, Wuhan 430071,China

    • Corresponding author: DONG Chang-yuan, 
    • Available online: 20 May 2006
    • Flow cytometry was used to quantify the percentage of apoptotic hepatic Caracimoma cells infected with bluetongue virus HbC3 and stained with propidium iodide (PI) and Annexin V/PI. The percentages of the apoptotic cells measured by PI at 24h, 36h and 48h were 10.8 ±3.05, 21.7±6.28, 28.3±10.6, respectively, and those by Annexin V-PI were 20.42±3.70, 49.3±8.11, 79.6±11.5, respectively. The two staining methods and the rate of apoptotic cells infected by bluetengue virus hours were significantly different (P0.01). The early apoptotic cells and secondary necrosis could be detected by AnnexinV/PI assay with good sensitivity and specificity. The apoptotic cells induced by bluetengue virus was one of the important forms in the pathological changes and cell necrosis.
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      Detection of Apoptosis of the Human Hepatic Carcinoma Cells Induced by Bluetongus Virus by PI and Annexin V/PI Staining Methods

        Corresponding author: DONG Chang-yuan,
      • 1. 1.Center of Medical Structural Biology, Wuhan University School of Medicine Wuhan University, wuhan 430071, China
      • 2. Laboratory of Virus and Cancer, Institute of Virology, Medical College, Wuhan University, Wuhan 430071,China

      Abstract: Flow cytometry was used to quantify the percentage of apoptotic hepatic Caracimoma cells infected with bluetongue virus HbC3 and stained with propidium iodide (PI) and Annexin V/PI. The percentages of the apoptotic cells measured by PI at 24h, 36h and 48h were 10.8 ±3.05, 21.7±6.28, 28.3±10.6, respectively, and those by Annexin V-PI were 20.42±3.70, 49.3±8.11, 79.6±11.5, respectively. The two staining methods and the rate of apoptotic cells infected by bluetengue virus hours were significantly different (P0.01). The early apoptotic cells and secondary necrosis could be detected by AnnexinV/PI assay with good sensitivity and specificity. The apoptotic cells induced by bluetengue virus was one of the important forms in the pathological changes and cell necrosis.

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