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Citation: LIU Hong-mei, QIN Ai-jian*, YE Jian-qiang, CHEN Hong-jun, SHAO Hong-xia, JIN Wen-jie, LIU Yue-long. Construction of Recombinant Marek’s Disease Virus Vaccine Strain CVI988/Rispens Expressing the VP2 Protein of IBDV [J].VIROLOGICA SINICA, 2006, 21(3) : 257-260.

Construction of Recombinant Marek’s Disease Virus Vaccine Strain CVI988/Rispens Expressing the VP2 Protein of IBDV

  • An expression cassette containing the VP2 gene of infectious bursal disease virus (IBDV) strain JS under the control of the CMV promoter and an enhancer in pcDNA3.1-VP2 was amplified by PCR and cloned into pUC18-US10 plasmid to yield the transferring vector pUC18-US10-VP2. The recombinant virus, designated rCVI988-VP2, was generated by co-transfection of CEF with pUC18-US10-VP2 and the Marek’s disease virus (MDV) vaccine CVI988/Rispens virus. PCR analysis and immunofluorenscence assays indicated that the recombinant virus was stable over 8 passages and that VP2 was expressed in CEF infected with the rCVI988-VP2. The data showed that the recombinant MDV expressing the VP2 gene of IBDV was successfully constructed and could prove to be a good strategy for IBD control.

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    Construction of Recombinant Marek’s Disease Virus Vaccine Strain CVI988/Rispens Expressing the VP2 Protein of IBDV

    • 1. Key Lab of Jiangsu preventive veterinary medicine, Yangzhou University, Yangzhou 225009, China

    Abstract: An expression cassette containing the VP2 gene of infectious bursal disease virus (IBDV) strain JS under the control of the CMV promoter and an enhancer in pcDNA3.1-VP2 was amplified by PCR and cloned into pUC18-US10 plasmid to yield the transferring vector pUC18-US10-VP2. The recombinant virus, designated rCVI988-VP2, was generated by co-transfection of CEF with pUC18-US10-VP2 and the Marek’s disease virus (MDV) vaccine CVI988/Rispens virus. PCR analysis and immunofluorenscence assays indicated that the recombinant virus was stable over 8 passages and that VP2 was expressed in CEF infected with the rCVI988-VP2. The data showed that the recombinant MDV expressing the VP2 gene of IBDV was successfully constructed and could prove to be a good strategy for IBD control.

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