For best viewing of the website please use Mozilla Firefox or Google Chrome.
Citation: HUANG Xin-xin, LU Cheng-ping*. Genome Sequencing and Structure Prediction of ZHZC3 Strain of Taura Syndrome Virus Isolated in China [J].VIROLOGICA SINICA, 2006, 21(3) : 267-272.

Genome Sequencing and Structure Prediction of ZHZC3 Strain of Taura Syndrome Virus Isolated in China

  • Eight overlapping clones covering the whole viral genome of TSV ZHZC3 isolate were amplified by RT-PCR and the 5′ and 3′ ends were amplified with RACE. The PCR products were cloned into pMD-18T vector and sequenced. The result showed that the genome of ZHZC3 consisted of 10202nt excluding the poly (A) tail, and contained two ORFS (ORFS1-2) which encoded polyproteins of 2107aa and 1011aa. No insertions or deletions were detected in the coding regions while 3nt of A were deleted at 5′ UTR when compared with HI94. The overall nucleotide sequence identity between ZHZC3 and HI94 was 97.6%. Compared with HI94 and BLZ01, the ORF1 of ZHZC3 shared 97.6% and 97.7% nucleotide sequence identity. The ORF2 shared 98.3 and 97% sequence identity at the nucleotide level. Compared with partial sequences of ORF2 from foreign isolates, both ZHZC3 and the Taiwan isolates shared higher nucleotide identity with HI94. Amino acid sequence analysis of six TSV isolates from different regions of mainland China revealed unique aa changes at 312 (S), 449 (A), 451 (Q), and 468 (H) in the highly variable region CP2 of ORF2, indicating that the isolates spread through the South-easten China most closely related to the other isolates with it’s own evolutionary character.

  • 加载中
  • 加载中

Article Metrics

Article views(2632) PDF downloads(968) Cited by()

Related
Proportional views
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Genome Sequencing and Structure Prediction of ZHZC3 Strain of Taura Syndrome Virus Isolated in China

    • 1. Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095

    Abstract: Eight overlapping clones covering the whole viral genome of TSV ZHZC3 isolate were amplified by RT-PCR and the 5′ and 3′ ends were amplified with RACE. The PCR products were cloned into pMD-18T vector and sequenced. The result showed that the genome of ZHZC3 consisted of 10202nt excluding the poly (A) tail, and contained two ORFS (ORFS1-2) which encoded polyproteins of 2107aa and 1011aa. No insertions or deletions were detected in the coding regions while 3nt of A were deleted at 5′ UTR when compared with HI94. The overall nucleotide sequence identity between ZHZC3 and HI94 was 97.6%. Compared with HI94 and BLZ01, the ORF1 of ZHZC3 shared 97.6% and 97.7% nucleotide sequence identity. The ORF2 shared 98.3 and 97% sequence identity at the nucleotide level. Compared with partial sequences of ORF2 from foreign isolates, both ZHZC3 and the Taiwan isolates shared higher nucleotide identity with HI94. Amino acid sequence analysis of six TSV isolates from different regions of mainland China revealed unique aa changes at 312 (S), 449 (A), 451 (Q), and 468 (H) in the highly variable region CP2 of ORF2, indicating that the isolates spread through the South-easten China most closely related to the other isolates with it’s own evolutionary character.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return