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Volume 21 Issue 3
June 2006
    Citation: YANG Wei-dong, ZHENG Yun, ZHANG Gui-ming, CHEN Zhi-nan, LI Ming-fu, YU Dao-jian, ZHANG Yong-jiang. Method for Detecting Tobacco Ringspot Virus by IC-RT-Realtime PCR .VIROLOGICA SINICA, 2006, 21(3) : 277-280.
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    Method for Detecting Tobacco Ringspot Virus by IC-RT-Realtime PCR

    • 1.

      1.Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518001, China

    • 2.

      Institute for Animal & Plant Quarantine of China Academy of Inspection and Quarantine, Beijing 100029, China

    • Corresponding author: YANG Wei-dong, 
    • Available online: 20 May 2006
    • The technique of immune capture reverse transcription real-time PCR (IC-RT-Realtime PCR) was applied for the first time to detect Tobacco ringspot virus. When detecting virus, it was shown to be advantageous in terms of specificity and sensitivity to integrate several techniques, such as specific bond of antigen and antibody, nucleic acid hybridization and real-time PCR over the traditional technique of DAS-ELISA. Application of the integrated techniques common problems such as masking of symptom, low concentration of virus and disturbing-materials.
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      Method for Detecting Tobacco Ringspot Virus by IC-RT-Realtime PCR

        Corresponding author: YANG Wei-dong,
      • 1. 1.Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518001, China
      • 2. Institute for Animal & Plant Quarantine of China Academy of Inspection and Quarantine, Beijing 100029, China

      Abstract: The technique of immune capture reverse transcription real-time PCR (IC-RT-Realtime PCR) was applied for the first time to detect Tobacco ringspot virus. When detecting virus, it was shown to be advantageous in terms of specificity and sensitivity to integrate several techniques, such as specific bond of antigen and antibody, nucleic acid hybridization and real-time PCR over the traditional technique of DAS-ELISA. Application of the integrated techniques common problems such as masking of symptom, low concentration of virus and disturbing-materials.

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