For best viewing of the website please use Mozilla Firefox or Google Chrome.
Citation: RAO Min, ZHANG Shu-ling, DONG Ji-hua, LI Shu-li. Inhibition of Hepatitis B Virus Replication by Homoharringtonine, IFN-α1b, Hydroxycamptothecin and Norfloxacin [J].VIROLOGICA SINICA, 2006, 21(3) : 284-287.

Inhibition of Hepatitis B Virus Replication by Homoharringtonine, IFN-α1b, Hydroxycamptothecin and Norfloxacin

  • Corresponding author: ZHANG Shu-ling, 
  • The inhibitory effects of homoharringtonine、IFN-α1b 、hydroxycamptothecin and norfloxacin on Hepatitis B virus(HBV)replication in vitro was investigated. Different concentrations of each drug were added to the culture medium of HepG2.2.15 cell line transfected with HBV. The Cytopathic effects(CPE)and cell toxicity of each drug was observed. Under the maximum non-toxic conentration(TC0),ELISA and fluorescent quantified PCR were used for determining HBsAg、HBeAg and HBVDNA in the culture medium. The results showed that homoharringtonine and IFN-α1b had an apparent inhibitory effect to HBV while Hydroxycamptothecinhad had only a weak effect to HBV replication. Norfloxacin had no inhibition effect to HBV at any concentration. This data t provide part of the experimental evidence needed to initiate clinical trials to evaluate homoharringtonine as an anti-HBV drub.

  • 加载中
  • 加载中

Article Metrics

Article views(2702) PDF downloads(908) Cited by()

Related
Proportional views
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Inhibition of Hepatitis B Virus Replication by Homoharringtonine, IFN-α1b, Hydroxycamptothecin and Norfloxacin

      Corresponding author: ZHANG Shu-ling,
    • 1. 1.Infectious Office,Union Hospital, Tong Ji Medical College of Huazhong University of Science and Technology,Wuhan 430022,China
    • 2. Virus Study Office, Union Hospital, Tong Ji Medical College of Huazhong University of Science and Technology,Wuhan 430022,China

    Abstract: The inhibitory effects of homoharringtonine、IFN-α1b 、hydroxycamptothecin and norfloxacin on Hepatitis B virus(HBV)replication in vitro was investigated. Different concentrations of each drug were added to the culture medium of HepG2.2.15 cell line transfected with HBV. The Cytopathic effects(CPE)and cell toxicity of each drug was observed. Under the maximum non-toxic conentration(TC0),ELISA and fluorescent quantified PCR were used for determining HBsAg、HBeAg and HBVDNA in the culture medium. The results showed that homoharringtonine and IFN-α1b had an apparent inhibitory effect to HBV while Hydroxycamptothecinhad had only a weak effect to HBV replication. Norfloxacin had no inhibition effect to HBV at any concentration. This data t provide part of the experimental evidence needed to initiate clinical trials to evaluate homoharringtonine as an anti-HBV drub.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return