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Citation: WANG Wei-hua, WANG Feng, CHEN Jie, WANG Li, ZHOU Xun-liang, GU Xin, ZHOU Bin-Lei, YU Hong-shen. Clinical Application of Flow Cytometry for Quantification of Hanta Virus Antigenemia in Patients with Hemorrhagic Fever with Renal Syndrome [J].VIROLOGICA SINICA, 2006, 21(3) : 298-300.

Clinical Application of Flow Cytometry for Quantification of Hanta Virus Antigenemia in Patients with Hemorrhagic Fever with Renal Syndrome

  • Corresponding author: CHEN Jie, 
  • The clinical value of the detection Hanta virus antigenemia in Peripheral blood mononuclear cells (PBMC) in patients with Hemorrhagic fever with renal syndrome was evaluated. Samples from 32 cases with varying degrees of HFRS were subjected to quantitative detection for HV membrane antigen in PBMC by flow cytometry. The correlation between clinical severity and positive rates was analyzed. On the first day of onset of illness, positive PBMC rates were highly different from group to group (P0.01), which suggested a close relationship between clinical severity and the positive rate. Dynamic observations showed that in the light and moderate groups, positive rates differed significantly from day to day. This was not the case in the severe group with no statistical significance between the 7th and 10th day(P0.05), which implied a relatively slow rate of virus elimination. Quantitative analysis of HV antigen in PBMC by FCM is a promising method in early diagnosis, individualized therapy and in evaluating the prognosis of HFRS.

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    Clinical Application of Flow Cytometry for Quantification of Hanta Virus Antigenemia in Patients with Hemorrhagic Fever with Renal Syndrome

      Corresponding author: CHEN Jie,
    • 1. 1.Clinical Laboratory Center, Affiliated Hospital of Medical College, Ningbo University, Ningbo 315020, China
    • 2. Medical Center of Ningbo City, Lihuili Hospital, Ningbo 315010, China
    • 3. Blood Center of Ningbo, Ningbo 315010, China
    • 4. Dart Mouth Medical College, USA

    Abstract: The clinical value of the detection Hanta virus antigenemia in Peripheral blood mononuclear cells (PBMC) in patients with Hemorrhagic fever with renal syndrome was evaluated. Samples from 32 cases with varying degrees of HFRS were subjected to quantitative detection for HV membrane antigen in PBMC by flow cytometry. The correlation between clinical severity and positive rates was analyzed. On the first day of onset of illness, positive PBMC rates were highly different from group to group (P0.01), which suggested a close relationship between clinical severity and the positive rate. Dynamic observations showed that in the light and moderate groups, positive rates differed significantly from day to day. This was not the case in the severe group with no statistical significance between the 7th and 10th day(P0.05), which implied a relatively slow rate of virus elimination. Quantitative analysis of HV antigen in PBMC by FCM is a promising method in early diagnosis, individualized therapy and in evaluating the prognosis of HFRS.

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