Citation: JU Hui-yan, XIA Xian-zhu, GAO Yu-wei, YANG Song-tao, ZOU Xiao-huan, HUANG Geng, LI Yan-fang. Construction and Identification of a Recombinant Canine Adenovirus Expressing Nucleocapsid Protein of Canine Distemper Virus .VIROLOGICA SINICA, 2006, 21(4) : 380-384.

Construction and Identification of a Recombinant Canine Adenovirus Expressing Nucleocapsid Protein of Canine Distemper Virus

  • Corresponding author: XIA Xian-zhu, 
  • Available online: 20 July 2006
  • The nucleocapsid protein gene (N) located 108 to 1679 situation of Canine distemper virus (CDV) genome and was immunogenicity protein with strong conservatism, so N gene was choosed as aim gene. A shuttle plasmid pVAXΔE3LPN containing the gene encoding the nucleocapsid protein of CDV was constructed by restriction endonuclease digestion and ligation. A recombinant plasmid pCAV-2-CDVLPN was constructed with pPoly2-CAV-2 vector containing CAV-2 SY genome. The plasmid pCAV-2-CDVLPN was transfected into MDCK cells by Lipofectamine and after three transfections, a typical CAV-2 cytopathic effect (CPE) was observed. We demonstrated that a recombinant canine adenovirus type-2 expressing nucleocapsid protein of canine distemper virus was successfully constructed and observed by electron microscopy, enzyme digestion, PCR and sequencing. The molecular mass of nucleocapsid protein was 58 kDa.

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    Construction and Identification of a Recombinant Canine Adenovirus Expressing Nucleocapsid Protein of Canine Distemper Virus

      Corresponding author: XIA Xian-zhu,
    • 1. 1.Institute of Military Veterinary, Academy of Military Medical Science of PLA, Changchun 130062, China
    • 2. Department of Agricultural Science, Jilin University, Changchun 130062, China

    Abstract: The nucleocapsid protein gene (N) located 108 to 1679 situation of Canine distemper virus (CDV) genome and was immunogenicity protein with strong conservatism, so N gene was choosed as aim gene. A shuttle plasmid pVAXΔE3LPN containing the gene encoding the nucleocapsid protein of CDV was constructed by restriction endonuclease digestion and ligation. A recombinant plasmid pCAV-2-CDVLPN was constructed with pPoly2-CAV-2 vector containing CAV-2 SY genome. The plasmid pCAV-2-CDVLPN was transfected into MDCK cells by Lipofectamine and after three transfections, a typical CAV-2 cytopathic effect (CPE) was observed. We demonstrated that a recombinant canine adenovirus type-2 expressing nucleocapsid protein of canine distemper virus was successfully constructed and observed by electron microscopy, enzyme digestion, PCR and sequencing. The molecular mass of nucleocapsid protein was 58 kDa.

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