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Volume 21 Issue 4
August 2006
    Citation: PENG Lu-bo, LI Ting-ting, WEI Chun-hong, FANG Qi, DONG Jia-hong, ZHANG Li-zhen, SU Xiao-xia, DING Ming, ZHANG Zhong-kai, LI Yi. Antiserum Preparation against Rice Dwarf Virus Kunming Isolate and Detection by Immunocapture PCR Tests .VIROLOGICA SINICA, 2006, 21(4) : 408-410.
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    Antiserum Preparation against Rice Dwarf Virus Kunming Isolate and Detection by Immunocapture PCR Tests

    • 1.

      1.The Biotechnology and Genetic Resources Institute，Yunnan Academy of Agricultural Sciences，Kunming 650223，China

    • 2.

      College of Life Science, Peking University, Beijing 100871, China

    • Available online: 20 July 2006
    • By Using the modified method to extract Rice dwarf virus virions from infected rice leaves, a concentration 3.854mg/mL of purified virus virions reached, and the Rabbits was immunized with purified virions, yielding high titer antiserum; 1:20480 by the indirect enzyme-linked immunosorbent assay (I-ELISA) test. The antiserum successfully detected three rice samples from Kunming, Yuxi and Luliang by I-ELISA.Immunocapture polymerase chain reaction (IC-PCR), respectively was the utilized to show that serologic and molecular methods were successfully used for detection of Rice dwarf virus.
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      Antiserum Preparation against Rice Dwarf Virus Kunming Isolate and Detection by Immunocapture PCR Tests

      • 1. 1.The Biotechnology and Genetic Resources Institute，Yunnan Academy of Agricultural Sciences，Kunming 650223，China
      • 2. College of Life Science, Peking University, Beijing 100871, China

      Abstract: By Using the modified method to extract Rice dwarf virus virions from infected rice leaves, a concentration 3.854mg/mL of purified virus virions reached, and the Rabbits was immunized with purified virions, yielding high titer antiserum; 1:20480 by the indirect enzyme-linked immunosorbent assay (I-ELISA) test. The antiserum successfully detected three rice samples from Kunming, Yuxi and Luliang by I-ELISA.Immunocapture polymerase chain reaction (IC-PCR), respectively was the utilized to show that serologic and molecular methods were successfully used for detection of Rice dwarf virus.

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