Expression and purification of HIV Integrase in E. col i and Application in HIV Diagnosis
Abstract: HIV-1 integrase P31 of RL42 strain, a B subtype isolate, was expressed in E.coli using pThioHisB vector(Invitrogen). The recombinant protein represented about 30% of total cellular proteins. and was purified by inclusion body washing and Ion Exchange Chromatography （IEX）. The purity of P31 protein reached more than 95%. Purified P31 antigen displayed fairly good sensitivity and specificity when prepared into immunoblot strips and tested with HIV reference sera. This work established a foundation for the development of HIV detection kits.