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Volume 21 Issue 5
October 2006
    Citation: LIU Lian-xing, HAO Yan-ling, YUAN Guo-liang, XU Bin, CHEN Pei, FU Jing-jing, LIU Yong, SHAO YI-ming. Expression and purification of HIV Integrase in E. col i and Application in HIV Diagnosis .VIROLOGICA SINICA, 2006, 21(5) : 413-416.
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    Expression and purification of HIV Integrase in E. col i and Application in HIV Diagnosis

    • 1.

      1. Wuhan Institute of Virology,Chinese Academy of Sciences, Wuhan 430071, China

    • 2.

      National Center for AIDS/STD Control and Prevention,Chinese Center for Disease Control and Prevention, Beijing 100050, China

    • 3.

      Graduate University of Chinese Academy of Sciences, Beijing 100049, China

    • 4.

      Hangzhou Ausia Bi-tech.Co.,Ltd, Hangzhou 310018, China

    • Available online: 20 September 2006
    • HIV-1 integrase P31 of RL42 strain, a B subtype isolate, was expressed in E.coli using pThioHisB vector(Invitrogen). The recombinant protein represented about 30% of total cellular proteins. and was purified by inclusion body washing and Ion Exchange Chromatography （IEX）. The purity of P31 protein reached more than 95%. Purified P31 antigen displayed fairly good sensitivity and specificity when prepared into immunoblot strips and tested with HIV reference sera. This work established a foundation for the development of HIV detection kits.
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      Expression and purification of HIV Integrase in E. col i and Application in HIV Diagnosis

      • 1. 1. Wuhan Institute of Virology,Chinese Academy of Sciences, Wuhan 430071, China
      • 2. National Center for AIDS/STD Control and Prevention,Chinese Center for Disease Control and Prevention, Beijing 100050, China
      • 3. Graduate University of Chinese Academy of Sciences, Beijing 100049, China
      • 4. Hangzhou Ausia Bi-tech.Co.,Ltd, Hangzhou 310018, China

      Abstract: HIV-1 integrase P31 of RL42 strain, a B subtype isolate, was expressed in E.coli using pThioHisB vector(Invitrogen). The recombinant protein represented about 30% of total cellular proteins. and was purified by inclusion body washing and Ion Exchange Chromatography （IEX）. The purity of P31 protein reached more than 95%. Purified P31 antigen displayed fairly good sensitivity and specificity when prepared into immunoblot strips and tested with HIV reference sera. This work established a foundation for the development of HIV detection kits.

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