Serving the Society Since 1986
Advanced Search
Volume 21 Issue 5
October 2006
    Citation: HUANG Xian-qing, BIE Xiao-mei, LU Zhao-xin, LV Feng-xia, YANG Shu-jing. In vitro Assays of an Antimicrobial Lipopeptide Produced by Bacillus subtilis fmbJ Against Pseudorabies Virus and Porcine Parvovirus .VIROLOGICA SINICA, 2006, 21(5) : 426-429.
    shu

    In vitro Assays of an Antimicrobial Lipopeptide Produced by Bacillus subtilis fmbJ Against Pseudorabies Virus and Porcine Parvovirus

    • 1.

      1.College of Food Science and Technology, Nanjing Agricultural University，Nanjing 210095, China

    • 2.

      College of Food schence and Technology, Henan Agricultural University, Zheng zhou 450002, China

    • Available online: 20 September 2006
    • The effect and mechanism of antimicrobial lipopeptide (AMI) produced by a Bacillus subtilis fmbJ strain against Pseudorabies virus (PRV) and Porcine parvovirus (PPV) were studied. The Median Toxicosis Dose (TD50) and TD0 values of AMI on Porcine Kidney (PK-15) cells were 47.57mg/L and 18.9 mg/L, respectively. AMI strongly inhibited the cytopathic effect induced by PRV and PPV, and increase the survival rate of cell remarkably. However, it did not inhibit infection and multiplication of PPV and PRV in PK-15 cells. This study indicated that the antimicrobial lipopeptide could affect PRV and PPV directly but precluded infection of PK-15 cells. the effect of AMI in PK-15 cells was markedly lower than the antiviral drug Acyclovir (ACV). Since it had lower toxicity to PK-15 cell, we would study it further as an antiviral drug.
    • 加载中
    • 加载中
    PDF

    Article Metrics

    Article views(4366) PDF downloads(1047) Cited by()

    Related
    Proportional views

      In vitro Assays of an Antimicrobial Lipopeptide Produced by Bacillus subtilis fmbJ Against Pseudorabies Virus and Porcine Parvovirus

      • 1. 1.College of Food Science and Technology, Nanjing Agricultural University，Nanjing 210095, China
      • 2. College of Food schence and Technology, Henan Agricultural University, Zheng zhou 450002, China

      Abstract: The effect and mechanism of antimicrobial lipopeptide (AMI) produced by a Bacillus subtilis fmbJ strain against Pseudorabies virus (PRV) and Porcine parvovirus (PPV) were studied. The Median Toxicosis Dose (TD50) and TD0 values of AMI on Porcine Kidney (PK-15) cells were 47.57mg/L and 18.9 mg/L, respectively. AMI strongly inhibited the cytopathic effect induced by PRV and PPV, and increase the survival rate of cell remarkably. However, it did not inhibit infection and multiplication of PPV and PRV in PK-15 cells. This study indicated that the antimicrobial lipopeptide could affect PRV and PPV directly but precluded infection of PK-15 cells. the effect of AMI in PK-15 cells was markedly lower than the antiviral drug Acyclovir (ACV). Since it had lower toxicity to PK-15 cell, we would study it further as an antiviral drug.

        HTML

      Relative (20)

      目录

      This journal is a member of, and subscribes to the principles of, the Committee on Publication Ethics (COPE)

      鄂ICP备05001977号

         

      鄂公网安备 42010602003674号

      Copyright © 2019 Virologica Sinica. All rights reserved

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return